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Master's Dissertation
DOI
https://doi.org/10.11606/D.21.2015.tde-10062015-152907
Document
Author
Full name
Natascha Menezes Bergo
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2015
Supervisor
Committee
Pellizari, Vivian Helena (President)
Brandini, Frederico Pereira
Lima, André Oliveira de Souza
Title in Portuguese
Abundância e distribuição espacial de micro-organismos na Plataforma Continental Sudeste e área oceânica adjacente
Keywords in Portuguese
Citometria de fluxo
Microbiologia Marinha
PCR em tempo real
Plataforma Continental Sudeste
Ressurgência
Abstract in Portuguese
A ressurgência da massa de Água Central do Altântico Sul (ACAS) é o principal processo oceanográfico de disponibilização de nutrientes na zona eufótica da plataforma continental sudeste do Brasil (PCSE). Isto gera um acréscimo na população de organismos autotróficos e consequente de heterotróficos que produzem e oxidam a matéria orgânica gerada. O que possibilita um aumento no fluxo de carbono e consequentemente a manutenção dos recursos pesqueiros na região durante o verão. A abundância de micro-organismos autotróficos e heterotróficos planctônicos e a relação destes com as massas de água da PCSE foram avaliadas no verão de 2013. Para o tal, os micro-organismos autotróficos, menores que 20 µm foram quantificados por citometria de fluxo. Foram realizadas análises de abundância do gene 16S de bactérias, archaeas e do clado Sar11 e a expressão dos genes rubisco e proteorodpsina por meio de PCR em tempo real. O valor médio obtido de micro-organismos autrotróficos, Prochlorococcus spp. e Synechococcus spp. foi de 15.151 e de 8.313 células.ml-1, respectivamente. E de bactérias heterotróficas de 7.4x105 células.ml-1. Em relação aos domínios, a distribuição de Bacteria ocorreu na superfície e de Archaea no fundo da região oceânica. Os resultados indicam que a distribuição dos micro-organismos ocorreu conforme as características físicas e a disponibilidade de nutrientes das massas de água. Bactérias heterotróficas foram abundantes em regiões costeiras provavelmente devido a maior disponibilidade de matéria orgânica, o que corrobora com o resultado encontrado de maior biomassa de carbono em região costeira.
Title in English
Abundance and spatial distribution of microorganisms in the Southeast Continental Shelf and adjacent ocean area
Keywords in English
Flow Cytometry
Marine Microbiology
Real Time PCR
Resurgence
Southeast Continental Shelf
Abstract in English
The resurgence of South Atlantic Central Waters (ACAS) is the main oceanographic process that involves availability of nutrients in the photic zone of the southeast continental shelf in Brazil. This causes an increase in the population of autotrophs and heterotrophic organisms (that produce and oxidizes the organic matter). The carbon flux increase and consequently the maintenance of fish resource in the region during the 2013 summer. The abundance of autotrophic and heterotrophic planktonic microorganisms was evaluated and also was studied their relation with the masses of water of the continental shelf. Thus, autotrophic microorganisms, smaller than 20 microns were quantified by flow cytometry. Using real time PCR were analysed 16S abundance of bacteria, archaea and SAR11 clade and the expression of the rubisco and proteorhodopsin gene. Autotrophic microorganisms, Prochlorococcus spp. and Synechococcus spp. had a mean value of 15.151 and 8.313 cell.ml-1, respectively. Heterotrophic bacteria had a mean value of 7.4x105 cell.ml-1. In relation to the domains, the distribution of bacteria occurred in the surface and the Archaea in the deep of the ocean region. The results indicate that the distribution of micro-organisms occur as the physical characteristics and the availability of nutrients of water masses. Heterotrophic bacteria were abundant in coastal areas probably due to increased of organic matter, which corroborates the result of higher biomass carbon in coastal region.
 
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Publishing Date
2015-06-10
 
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