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Master's Dissertation
DOI
https://doi.org/10.11606/D.18.2007.tde-02072007-230623
Document
Author
Full name
Tiago Henrique Martins
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Carlos, 2007
Supervisor
Committee
Silva, Maria Bernadete Amancio Varesche (President)
Foresti, Eugenio
Tommaso, Giovana
Title in Portuguese
Enriquecimento de consórcios microbianos em quimiostatos sob condições anammox
Keywords in Portuguese
Anammox
DGGE
FISH
Nitrogênio
Quimiostatos
Abstract in Portuguese
Esta pesquisa objetivou enriquecer e purificar, em quimiostatos, consórcios microbianos capazes de oxidar amônia a nitrogênio ('N IND.2'), sob condições anaeróbias utilizando como inóculo: (Q1) biomassa de reator nitrificante-desnitrificante de estação de tratamento de água residuária de indústria produtora de aminoácidos (Ajinomoto) e (Q2) lodo granular de reator UASB de abatedouro de aves (Avícola DACAR, TIETÊ). Os inóculos foram enriquecidos em quimiostatos com vazão afluente de 18ml/h de meio basal específico com concentrações médias de 80 mg 'N-NH IND.4'POT.+'/L, 75,2 mg 'N-NO IND.2'POT.-'/L e 1000 mg/l de bicarbonato como única fonte de carbono. Nos quimiostatos Q1 e Q2 as eficiências médias de remoção foram de 51,6% e 39,3% de amônia e 60,5% e 53,2% de nitrito, respectivamente, após 296 dias de operação. A eficiência média de remoção de nitrogênio total ('N-NH IND.4'POT.+' + 'N-NO IND.2'POT.-') foi de 57,1% e 43% após 296 dias de operação, respectivamente para Q1 e Q2. A purificação dos consórcios enriquecidos foi realizada utilizando-se metodologia de gradiente de densidade por centrifugação Percoll. Os consórcios microbianos foram observados por exames microscópicos e a diversidade foi avaliada por DGGE antes e depois da purificação pelo protocolo Percoll. O DGGE revelou mudança na estrutura dos consórcios presentes em Q1 e Q2 no decorrer do período de operação dos quimiostatos. A técnica de hibridação in situ (FISH) com sonda fluorescente (Amx - 368) confirmou a presença de microrganismos anammox nos dois consórcios microbianos. O seqüenciamento do DNA ribossomal 16S de bandas obtidas do gel de DGGE, utilizando-se primers universais para domínio Bacteria, relacionou por árvore de máxima verossimilhança, duas bandas com o grupo das bactérias verdes não-sulfurosas.
Title in English
Enrichment of microbial trusts in chemostats with anammox conditions
Keywords in English
Anammox
Chemostats
DGGE
FISH
Nitrogen
Abstract in English
This research aimed to enrich and to purify, in chemostats, microbial trusts capable to realize ammonium oxidation to dinitrogen ('N IND.2') under anaerobic conditions, using as inoculum: (Q1) biomass from nitrifying-denitrifying reactor of wastewater treatment plant of amino-acids industry (Ajinomoto) and (Q2) granular sludge from upflow anaerobic sludge blanket UASB reactor treating poultry wastes - Avícola DACAR, TIETÊ. The inoculum was enriched in chemostats with affluent flow of 18 ml/h of specific basal media with mean concentrations of 80 mg 'N-NH IND.4'POT.+'/L, 75.2 mg 'NO IND.2'POT.-'/L and 1000 mg/l of bicarbonate as sole carbon source. In Q1 and Q2 chemostats the mean efficiency of removal were 51.6% and 39.3% of ammonium, and 60.5% and 53.2% of nitrite, respectively, after 296 days of operation. In Q1 and Q2 chemostats, the mean efficiency of total nitrogen removal ('N-NH IND.4'POT.+' + 'NO IND.2'POT.-') were 57.1% and 43%, respectively, after 296 days of operation. The purification of enriched microbial trusts was carried following methodology of density gradient by centrifugation (Percoll). The microbial trusts were observed by microscopic analysis and the diversity was evaluated by DGGE, before and after the purification by the Percoll protocol. The DGGE analysis showed changes in microbial trusts structure in Q1 and Q2 in chemostats operation period. The fluorescence in situ hibridization technique (FISH) with Amx-368 probe confirmed the presence of anammox microrganisms in both microbial trusts. The sequencing of recovered bands of DGGE was carried through ribossomal DNA 16S using universal primers for bacteria Domain that related two bands with green nosulphur bacterium using maximum likelyhood tree.
 
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MARTINSTH.pdf (4.09 Mbytes)
Publishing Date
2007-07-04
 
WARNING: The material described below relates to works resulting from this thesis or dissertation. The contents of these works are the author's responsibility.
  • MARTINS, T. H., e Varesche, M. B. Influência de micronutrientes na nitrificação. In XVII Simpósio Nacional de Bioprocessos - SINAFERM, Natal/RN, 2009. XVII SINAFERM., 2009.
  • MARTINS, T. H., GUSMÃO, V. R., e VARESCHE, M. B. A. Caracterização nutricional de culturas desnitrificantes na presença de benzeno e etilbenzeno. In IX Taller y Simposio Latinoamericano de Digestión Anaeróbia, Ilha de Páscoa, 2008. DAAL., 2008.
  • MARTINS, Tiago Henrique, et al. Enriquecimento, purificação e caracerização de cultura desnitrificante na presença de benzeno. In Sinaferm - XV Simpósio Nacional de Bioprocessos, Recife - Peranmbuco, 2005. Sinaferm - XV Simpósio Nacional de Bioprocessos., 2005.
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