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Master's Dissertation
DOI
10.11606/D.17.2006.tde-13022007-141641
Document
Author
Full name
Fabiana Ribeiro do Vale
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Ribeirão Preto, 2006
Supervisor
Committee
Silva, Alfredo Ribeiro da (President)
Arias, Victor Eduardo Arrua
Carrara, Helio Humberto Angotti
Title in Portuguese
Fatores reguladores da angiogênese e infiltração tumoral nos carcinomas mamários positivos para a citoqueratina 5
Keywords in Portuguese
angiogênese
carcinoma mamário
citoqueratina 5
infiltração tumoral
Abstract in Portuguese
O reconhecimento de subtipos de carcinomas mamários baseados em suas características moleculares trouxe novas perspectivas na investigação do câncer de mama. Algumas proteínas chaves reguladoras da angiogênese e da infiltração tumoral foram avaliadas em carcinomas de mama de fenótipo basal (CK5+). Foi realizado estudo Imunoistoquímico com 14 anticorpos primários em 100 casos de carcinoma ductal. A positividade para citoqueratina 5 correlacionou-se com indicadores de mau prognóstico, incluindo idade precoce, alto grau histológico, linfonodos positivos, estádio patológico avançado, negatividade para receptores hormonais, e uma alta taxa de proliferação celular, avaliado pelo Ki67. A positividade para a CK5 também se correlacionou com a expressão do VEGF, mas não com a densidade da microvascularização. Considerando que a superexpressão de VEGF pelas células neoplásicas da mama leva a um aumento da atividade proliferativa in vitro independente de seu efeito angiogênico, a expressão diferencial do VEFG pode contribuir para o comportamento mais agressivo da neoplasia. Houve correlação do CK5 com TIMP1, mas não com MMP1, MMP2, EMMPRIN, TIMP2 and PAI, indicando que o estimulo antiproteolítico pode ser preponderante nesta neoplasia.
Title in English
Regulating factors of angiogênese and tumoral infiltration in positive the mammary carcinomas for citoqueratina 5
Keywords in English
3 Metalloproteinases
4 Breast Carcinoma
Angiogenesis
Cytokeratin 5
Abstract in English
The recognition of subtypes of breast carcinomas based on their molecular features has brought new perspectives in breast cancer investigation. Some key regulators of angiogenesis and tumor infiltration were evaluated in breast carcinomas of basal-phenotype (CK5+). Immunohistochemistry with 14 primary antibodies was performed in 100 formalin-fixed paraffin-embedded samples of invasive ductal carcinomas. Cytokeratin 5 correlated with indicators of poor outcome, including precocious age, high histological grade, lymph node positivity, advanced pathological stage, negativity for hormonal receptors, and a high proliferative rate (Ki67 labeled index). CK5 also correlated with VEGF expression but not with the microvessel density. Considering that VEGF overexpressing neoplastic mammary cells display increased proliferative activity in vitro regardless its angiogenic effect, the differential expression of VEGF may contribute for the more aggressive behavior of these neoplasms. CK5 correlated with TIMP1, but not MMP1, MMP2, EMMPRIN, TIMP2 and PAI, indicating that anti-proteolytic stimuli may be preponderant in these neoplasms.
 
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Fabiana.pdf (330.73 Kbytes)
Publishing Date
2007-03-17
 
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