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Doctoral Thesis
DOI
https://doi.org/10.11606/T.17.2006.tde-06032007-125512
Document
Author
Full name
Vanessa Vilela Monte Raso
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Ribeirão Preto, 2006
Supervisor
Committee
Barbieri, Claudio Henrique (President)
Fazan, Valeria Paula Sassoli
Fonseca, Marisa de Cássia Registro
Holanda, Adriano de Jesus
Rocha, Murilo Antônio
Title in Portuguese
Índice funcional do ciático nas lesões por esmagamento do nervo ciático de ratos. Avaliação da reprodutibilidade do método entre examinadores
Keywords in Portuguese
avaliação funcional
índice funcional do ciático
interexaminadores.
lesão por esmagamento
nervo ciático de ratos
Regeneração de nervo periférico
reprodutibilidade do método
Abstract in Portuguese
Foi avaliada a reprodutibilidade entre examinadores do método de avaliação do Índice Funcional do Ciático (IFC), medido por um programa de computação desenvolvido para este fim. Foram empregados 20 ratos Wistar, cujo nervo ciático direito era abordado sob anestesia geral e esmagado num segmento de 5 mm proximal à sua trifurcação com um dispositivo especial, com carga fixa de 5 kgf por 10 minutos. Impressões das pegadas dos animais foram obtidas na fase pré-operatória e, depois, semanalmente, da 1ª à 8ª semana pós-operatória, em uma pista de marcha. As impressões eram digitalizadas, armazenadas e avaliadas, pela medida de parâmetros pré-determinados, por quatro examinadores, seguindo sempre a mesma seqüência de marcação dos parâmetros. Os resultados foram submetidos à análise estatística, que mostrou haver um alto índice de correlação entre examinadores na avaliação pré-operatória e nas 3a, 4a, 5a, 7a e 8a semanas (igual ou maior que 0,82), com queda casual na 6a semana, mas manteve significante como as demais (pF?0,01). Na 1a e 2a semanas, o índice de correlação foi próximo de zero, mostrando a pouca reprodutibilidade do método nesse período, em que a variabilidade entre os animais não diferiu da variabilidade entre os examinadores (pF?0,24 e 0,32, respectivamente), devido à pouca definição das impressões das pegadas.
Title in English
Sciatic functional index assessment to evaluate functional recovery following a crush injury of the sciatic nerve of rats. Inter-personal reproducibility of the method
Keywords in English
crush injury
functional evaluation
interexaminer reproducibility.
multiple examiners
sciatic functional index
Sciatic nerve of rats
Abstract in English
The Sciatic Functional Index (SFI) is a quite useful tool to evaluate functional recovery of the sciatic nerve of rats in a number of experimental lesions and treatment. Although being an objective method, it depends on the examiner?s ability to adequately recognize and mark the previously established footprint landmarks, which is an entirely subjective step, thus potentially interfering with the calculations according to the mathematical formulae proposed by different authors. An inter-personal evaluation of the reproducibility of an SFI computer aided method was then carried out here to study data variability. A severe crush injury was produce on a 5 mm-long segment of the right sciatic nerve of 20 Wistar rats (5kgf load directly applied for 10 minutes) and the SFI was measured by four different examiners (2 experienced and 2 new-comers) either preoperatively and at weekly intervals from the 1st to the 8th postoperative week. Three measurements were made for each print and an average was drawn and used for the statistical analysis. The results showed that interexaminer correlation was high (0.82) on the 3rd, 4th, 5th, 7th and 8th weeks, with an unexpected (pF?0.01) drop on the 6th week. There was virtually no inter-personal correlation (correlation index nearly 0) on the 1st and 2nd weeks, period in which the variability between animals (pF?0.24) and examiners (pF?0.32) was similar, certainly due to the poor definition of the footprints. The authors conclude that the SFI method studied here is only reliable from the 3rd week on.
 
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Publishing Date
2008-07-15
 
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