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Doctoral Thesis
DOI
https://doi.org/10.11606/T.17.2003.tde-18032007-140804
Document
Author
Full name
Adriana Gonela
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Ribeirão Preto, 2003
Supervisor
Committee
Contel, Eucleia Primo Betioli (President)
Lama, Silvia Nassif Del
Lui, Jeffrey Frederico
Manfrin, Maura Helena
Simões, Aguinaldo Luiz
Title in Portuguese
Aplicação de marcadores microssatélites de Sus scrofa domestica na caracterização genética de populações de Sus scrofa sp (porco-monteiro) e Tayassu pecari (queixada)
Keywords in Portuguese
amplificação heteróloga
microssatélites
porco-Monteiro
queixada
Abstract in Portuguese
Os microssatélites ou SSR (Simple Sequence Repeat) por serem considerados os marcadores moleculares ideais, têm se tornado o suporte principal nas análises genômicas em diferentes organismos. Neste estudo, as freqüências alélicas de seis locos microssatélites desenvolvidos para Sus scrofa domestica (ACTG2, ALOX12A, CGA, IGF1, SW444 e TNFB) foram estimadas em duas espécies de Suiformes, Sus scrofa sp e Tayassu pecari. Foi utilizada uma amostra representativa de 99 indivíduos (85% de T. pecari e 15% de S. scrofa sp). Os marcadores foram altamente variáveis, mostrando entre 4 e 15 alelos. A heterozigose observada variou de 0,13 a 1,00 e o conteúdo de polimorfismo informativo de 0,18 a 0,84. Estes resultados indicaram a conservação entre as espécies de suínos e demonstraram a viabilidade da utilização de iniciadores desenvolvidos para S. s. domestica na análise de outras espécies de Suiformes.
Title in English
Application of marking microssatélites of Sus scrofa domesticates in the genetic characterization of populations of Sus scrofa sp (Porco-Monteiro) and Tayassu pecari (jaw)
Keywords in English
heteróloga amplification
jaw
microssatélites
Porco-Monteiro
Abstract in English
Microsatellites have been considered a superior class of genetic markers over earlier ones and became the most useful for genomic population analysis in a great variety of organisms. In this paper we had estimated genetic frequencies of six STRs (ACTG2, ALOX12A, CGA, IGF1, SW444 and TNFB) developed for Sus scrofa domestica that were used to amplify markers in two natural populations of a neotropical feral Suidae (Sus scrofa sp) and a neotropical species of peccary (Tayassu pecari). High degree of polymorphisms was observed and the number of alleles varied from 4 to 15 for the six analyzed loci. The observed heterozygosity was between 0.13 to 1.00 and PIC values varied from 0.18 to 0.84. The study also showed that these markers are evolutionary conserved, allowing the possibility of heterologous amplification.
 
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AdrianaGonela.pdf (1.05 Mbytes)
Publishing Date
2008-02-29
 
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