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Doctoral Thesis
DOI
https://doi.org/10.11606/T.11.2014.tde-02062014-114207
Document
Author
Full name
Weliton Dias da Silva
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 2014
Supervisor
Committee
Bento, José Maurício Simões (President)
Godoy, Wesley Augusto Conde
Guerreiro Filho, Oliveiro
Pare, Paul Whitaker
Parra, José Roberto Postali
Title in Portuguese
Idade de fêmeas colonizadoras de Hypothenemus hampei (Ferrari) e sua resposta aos voláteis de flores de café, Coffea arabica L.
Keywords in Portuguese
Bioecologia
Broca-do-café
Ecologia Química
Scolytinae
Semioquímicos
Abstract in Portuguese
A idade das fêmeas colonizadoras de Hypothenemus hampei (Ferrari) durante o abandonamento do hospedeiro, assim como sua resposta aos voláteis de flores de café, Coffea arabica L. var. Tupi, foram investigadas neste trabalho. Um dispositivo experimental que simulasse as condições de dentro do fruto de café e permitisse a observação da saída dos insetos foi utilizado para a determinação da idade das fêmeas colonizadoras. Em média, os besouros apresentaram 15 dias de idade no momento em que abandonaram o dispositivo experimental. Nesta idade as fêmeas estavam acasaladas, possuiam o tegumento totalmente melanizado, foram capazes de voar e produzir ovos viáveis. Os voláteis de flores de café, foram coletados por aeração, analisados por GC-EAD e GC-MS, e testados em bioensaios olfatométricos. As fêmeas colonizadoras de H. hampei foram atraídas pelos voláteis de flores de café. Dos 50 compostos encontrados nos extratos naturais, sete foram eletrofisiologicamente ativos aos insetos. Destes sete, somente metil salicilato, neral e geranial puderam ser identificados, e a mistura de seus padrões sintéticos foi a mais atrativa nos bioensaios olfatométricos. Tomados juntos, estes resultados trazem novas informações sobre a bioecologia e ecologia química da broca-do-café, as quais poderão ser usadas futuramente em pesquisas de base e naquelas focadas no desenvolvimento de novas estratégias de manejo para H. hampei em cafezais.
Title in English
Age of colonizing Hypothenemus hampei (Ferrari) females and their response to volatiles of coffee, Coffea arabica L., flowers
Keywords in English
Bioecology
Chemical Ecology
Coffee berry borer
Scolytinae
Semiochemicals
Abstract in English
The age of Hypothenemus hampei (Ferrari) females during the host abandonment, as well as their response to coffee flower volatiles were determinated under laboratory conditions. A experimental device that simulated the conditions inside a coffee berry, and that allowed the observation the insects coming out was used to determinate the age of colonizing females. On average, the beetles were 15 d-old at the moment they abandoned the experimental device. In this age, the females were mated, had a fully melanized tegument, were able to fly and to produce viable eggs. The volatiles of coffee, Coffea arabica L. var. Tupi, flowers were colected by aeration, analyzed in GC-EAD and GC-MS, and tested in olfactometer bioassays. The colonizing H. hampei females were attracted by the coffee flower volatiles. Of 50 compounds found in the natural extracts, seven were electrophysiologically actives to the insects. Of these seven, only methyl salicylate, neral and geranial could be identified, and the blend with their syntetic standards was the most atractive in the olfactometer bioassays. Taken together, these results bring new information on the bioecology and chemical ecology of the coffee berry borer, which may be used in the future by basic researchs or by those focused on the development of new strategies for the management of H. hampei in coffee farms.
 
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Publishing Date
2014-06-10
 
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