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Master's Dissertation
DOI
10.11606/D.11.2006.tde-08032006-141346
Document
Author
Full name
Henrique Sergio Alves
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 2005
Supervisor
Committee
Carrer, Helaine (President)
Carraro, Dirce Maria
Labate, Carlos Alberto
Title in Portuguese
Sequenciamento e análise do genoma cloroplastidial de eucalipto (Eucalyptus grandis)
Keywords in Portuguese
cloroplastos vegetais
eucalipto
genomas
melhoramento genético
seqüência de DNA
vegetal
Abstract in Portuguese
Os cloroplastos encontrados nas folhas de plantas e algas pertencem a uma classe de organelas subcelulares denominadas de plastídios. Os plastídios possuem seu próprio genoma (plastoma), o qual contêm genes que participam de funções essenciais no metabolismo vegetal, como fotossíntese, síntese de aminoácidos e outras rotas biossintéticas. O plastoma da maioria das plantas superiores tem tamanho entre 120 a 180 kb. Em angiospermas é caracterizado pela presença de duas regiões repetidas invertidas (IRs) separadas por duas regiões de cópia única; uma longa (LSC) e outra curta (SSC). Para o sequenciamento completo da molécula do DNA (cpDNA) de Eucalyptus grandis, foram preparadas bibliotecas que geraram 9.033 seqüências de DNA. A seqüência completa foi determinada e possui o tamanho de 160.292 pb. As regiões IRs apresentam ter 26.400 pb; a SSC, 18.501 pb e; a LSC, 88.991 pb. As regiões codificadoras foram anotadas por análise de similaridade ao plastoma de Eucalyptus globulus. Todas as categorias gênicas presentes neste plastoma foram encontradas no plastoma de E. grandis. Estas duas espécies possuem 99,57% de similaridade na seqüência de nucleotídeos e apresentam total similaridade na organização dos seus genes. A disponibilidade de plastomas completos de diferentes espécies de Eucalyptus, torna possível realizar estudos comparativos para a identificação de polimorfismos espécie-específicos, importantes para serem utilizados como marcadores moleculares no melhoramento genético de Eucalyptus.
Title in English
Sequencing and analysis of eucalyptus (Eucalyptus grandis) chloroplast genome
Keywords in English
chloroplast
DNA sequence
eucalyptus
genetic breeding
genome
vegetable
Abstract in English
The chloroplasts found in plant leaves and algae belong to a class of subcellular organelle called plastids. The plastids has its own genome (plastome) which contain genes that participate in mainly functions on plant metabolism like photosynthesis, amino acids synthesis and other biosynthetic pathways. The plastome of most of higher plants are between 120-180 kb in size. It is characterized in angiosperms by two inverted repeat regions (IRs) separated by two regions of unique single copies; one large (LSC) and another small (SSC). For the complete sequencing of the DNA (cpDNA) molecule of chloroplast from Eucalyptus grandis, libraries were prepareted and generated 9,033 DNA sequences. The complete sequence was determined and it is 160,292 bp in size. The IRs showed to have 26,400 bp; the SSC, 18,502 bp and; the LSC, 88.991 bp in size. The gene coding regions were annotated by similarity analysis against the Eucalyptus globulus plastome. All types of gene categories present in E. globulus were found in E. grandis plastome. These two species show 99.57% of similarity on nucleotide sequence and they both present total similarity in their gene organization. The availability of complete plastomes from different Eucalyptus species make possible to perform comparative studies to identify species-specific polymorphisms, wich are important to be used as molecular markers in Eucalyptus breeding programs.
 
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HenriqueAlves.pdf (372.32 Kbytes)
Publishing Date
2006-03-22
 
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