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Master's Dissertation
DOI
10.11606/D.11.2002.tde-29102002-161542
Document
Author
Full name
Maria Cecília da Silva
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 2002
Supervisor
Committee
Gallo, Claudio Rosa (President)
Angelis, Dejanira de Franceschi de
Oetterer, Marilia
Title in Portuguese
Avaliação da qualidade microbiológica de alimentos com a utilização de metodologias convencionais e do sistema simplate.
Keywords in Portuguese
microbiologia de alimentos
microrganismo
qualidade dos alimentos
Abstract in Portuguese
Com a finalidade de se avaliar a correlação entre o sistema SimPlate e metodologias convencionais na análise microbiológica de alimentos, dezoito alimentos (nove de origem vegetal e nove de origem animal) foram analisados para contagem total de mesófilos aeróbios, bolores e leveduras e coliformes totais e fecais. Os resultados das análises microbiológicas foram comparados através do teste F, tendo havido diferença significativa (p<0,05) entre os métodos. As médias foram submetidas ao teste t-Student (p<0,05); para a contagem total de mesófilos aeróbios apenas em dois dos quinze alimentos considerados, maçã e beterraba, as metodologias convencional e SimPlate não diferiram entre si; para bolores e leveduras as metodologias não diferiram em quatro dos treze alimentos considerados, lingüiça, peixe, cheiro-verde e beterraba, e para coliformes totais, em seis dos doze alimentos considerados, carne suína, frango, lingüiça, leite cru, pêra e tomate. Uma análise de regressão dos dados mostrou boa correlação entre os dados obtidos para coliformes totais, pela metodologia dos tubos múltiplos e o SimPlate (0,88) e os dados de mesófilos aeróbios, obtidos pelo PCA e o SimPlate (0,80) e baixa correlação entre os dados obtidos na determinação de bolores e leveduras pelo BDA e o SimPlate (0,69).
Title in English
Utilization of conventional methodologies and simplate system for microbiological quality evaluation of foods.
Keywords in English
food microbiology
food quality
microorganism
Abstract in English
In order to evaluate correlation among SimPlate system and conventional methods for food microbiological analysis, eighteen foods (nine from vegetable origin and nine from animal origin) were analyzed for mesophilic aerobic, yeasts and molds, total coliforms and fecal coliforms. Results of the three analysis were compared and significant differences (p<0,05) occurred among the majority of foods. For mesophilic aerobic counts, between the methodologies, PCA and SimPlate, difference were not significant in two of fifteen considered foods, apple and beetroot; for yeasts and molds, using acidify PDA and SimPlate, difference were not significant in four of thirteen considered foods, mixed sausage, fish, parsley and Welsh onion and beetroot; for total coliforms, using three-tube method and SimPlate, difference were not significant in six of twelve considered foods, raw pork, chicken, mixed sausage, raw milk, pear and tomato. Regression analysis of data showed correlation coefficients like, 0,88 for three-tube method and SimPlate; 0,80 between PCA and SimPlate and 0,69, between acidify PDA and SimPlate.
 
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Publishing Date
2002-10-30
 
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