• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Master's Dissertation
DOI
https://doi.org/10.11606/D.11.2010.tde-24022010-103823
Document
Author
Full name
Luciane Santini
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 2009
Supervisor
Committee
Vieira, Maria Lucia Carneiro (President)
Brondani, Rosana Pereira Vianello
Garcia, Antonio Augusto Franco
Title in Portuguese
Mapeamento de genes análogos de resistência a patógenos em feijão (Phaseolus vulgaris L.)
Keywords in Portuguese
Feijão
Fungos fitopatogênicos
Genes
Mancha angular
Mapeamento genético
Marcador molecular
Oídio
Resistência genética vegetal.
Abstract in Portuguese
No presente trabalho, a metodologia NBS-profiling foi utilizada para o desenvolvimento de marcadores RGA (Resistance Genes Analogs) em duas populações de Phaseolus vulgaris, sendo uma derivada do cruzamento entre Bat 93 e Jalo EEP558 (BJ) e a outra derivada do cruzamento entre Carioca e Flor de Mayo (CFM). Uma vez identificados, foram mapeados 32 marcadores RGA na população BJ e 40 na população CFM. Nove dos marcadores alocados no mapa de ligação da população BJ se localizaram em proximidade a clusters de resistência, já identificados por outros pesquisadores. Foi realizado o sequenciamento de 32 dos RGA detectados, sendo 16 da cada população. Cinco sequências oriundas da população BJ e três sequências da população CFM apresentaram similaridade com proteínas de resistência identificadas em P. vulgaris, Glycine max e Medicago truncaluta. O mapa de ligação aqui gerado para a população CFM foi utilizado para o posicionamento de QTL (Quantitative Trait Loci) de resistência à mancha-angular (Pseudocercospora griseola (Sacc.) Crous & Braun) e ao oídio (Erysiphe polygoni DC.). Um total de 12 QTL foi mapeado, cinco associados à resposta à mancha angular e sete à resposta ao oídio.
Title in English
Mapping resistance gene analogs in common bean (Phaseolus vulgaris L.)
Keywords in English
Angular leaf spot
Common bean
Genes
Molecular markers
Phytopathogenic fungi
Plant genetic resistance.
Powdery mildew
Abstract in English
In the presenty study, the NBS-profiling method was used for the development of RGA (Resistance Gene Analogs) markers in two populations of Phaseolus vulgaris, one derived from a cross between 'Bat 93' and 'Jalo EEP558' (BJ) and the other derived from a cross between 'Carioca' and 'Flor de Mayo (CFM). After their identification, 32 RGA markers were mapped on the BJ population and 40 on the CFM population. Nine of the markers assigned to the linkage map of the BJ population were located in the proximity to clusters of resistance already identified by other researchers. We carried out the sequencing of 32 out of the RGA detected, being 16 from each population. Five sequences derived from the BJ population and three sequences from the CFM population showed similarity to resistance proteins identified in P. vulgaris, Glycine max and Medicago truncaluta. The linkage map here generated for the CFM population was used for the positioning of QTL (Quantitative Trait Loci) for resistance to angular leaf spot (Pseudocercospora griseola (Sacc.) Crous & Braun) and powdery mildew (Erysiphe polygoni DC.). Twelve QTL were mapped, five associated to the response to the angular leaf spot and seven to the powdery mildew.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Luciane_Santini.pdf (13.82 Mbytes)
Publishing Date
2010-03-05
 
WARNING: Learn what derived works are clicking here.
All rights of the thesis/dissertation are from the authors
CeTI-SC/STI
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2024. All rights reserved.