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Doctoral Thesis
DOI
https://doi.org/10.11606/T.11.2015.tde-22062015-174406
Document
Author
Full name
Milca Bartz Vilhena
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 2015
Supervisor
Committee
Azevedo, Ricardo Antunes de (President)
Borgo, Lucelia
Carvalho, Rogério Falleiros
Molina, Silvia Maria Guerra
Ribeiro, Manuella Nóbrega Dourado
Title in Portuguese
Diferentes abordagens para o estudo da resposta de solanáceas ao Cd
Keywords in Portuguese
Cádmio
Enxertia
Estresse oxidativo
Pimenta
Sistema antioxidante
Tomateiro
Abstract in Portuguese
O metal pesado cádmio (Cd) é um contaminante ambiental de grande relevância devido aos vários problemas que pode causar para a agricultura, como perdas na produção e na qualidade dos alimentos, podendo oferecer riscos à saúde humana através do consumo de vegetais contaminados. Neste trabalho abordamos duas formas diferentes e independentes para o estudo do estresse oxidativo. Exploramos métodos para compreender melhor: a interação do Cd com o sistema antioxidante de pimentas; bem como os mecanismos responsáveis pela tolerância ao metal utilizando tomateiros com diferentes graus de tolerância em diferentes combinações de enxertia. Resultados interessantes foram obtidos para a combinação de uma variedade sensível enxertada em uma variedade tolerante ao Cd, que apresentou melhor crescimento enxertada em relação a cada uma das variedades enxertadas como controle, inclusive na presença de Cd. Tais resultados podem indicar novos caminhos de pesquisa. No estudo com a pimenta também encontramos respostas notáveis com relação ao sistema antioxidante, em especial, na resposta do ciclo ascorbato-glutationa, cujos compostos antioxidantes foram os principais protagonistas na defesa da planta contra o estresse por Cd.
Title in English
Different approaches to study the responses to Cd stress in Solanaceae
Keywords in English
Antioxidant system
Cadmium
Grafting
Oxidative stress
Pepper
Tomato
Abstract in English
The heavy metal cadmium (Cd) is an environmental contaminant of great relevance because this element can cause several problems to agriculture, such as production decrease and poor food quality, thereby posing risk to human health through consumption of contaminated vegetables. In this research, we used two different and independent approaches to study the oxidative stress. We explored methods: to better understand Cd interaction with the antioxidant system of pepper; as well as the mechanisms responsible for conferring tolerance to this metal, using tomatoes with different degrees of tolerance grafted in different combinations. Interesting results were obtained, especially when the sensitive tomato line was grafted onto the most tolerant one, presenting a better growth index even in the presence of Cd. These results might lead to new research alternatives and approaches. The results from the study with pepper revealed noteworthy responses regarding the antioxidant system, especially in the ascorbate-glutathione cycle response, whose antioxidant compounds were the main protagonists in the plant defense against stress by Cd.
 
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Publishing Date
2015-07-01
 
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