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Master's Dissertation
DOI
https://doi.org/10.11606/D.11.2020.tde-05052020-083432
Document
Author
Full name
Mariana Shizue Gouveia Saito
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 2020
Supervisor
Committee
Kitajima, Elliot Watanabe (President)
Novelli, Valdenice Moreira
Tassi, Aline Daniele
Title in Portuguese
O vírus da pinta verde do maracujazeiro (passion fruit green spot virus - PfGSV), isolado de ligustro: suas relações com o ácaro vetor Brevipalpus
Keywords in Portuguese
Ácaros Brevipalpus
PfGSV-Lig
Relações vírus vetor
VTBs
Abstract in Portuguese
O vírus da pinta verde do maracujá (passion fruit green spot virus- PfGSV), transmitido por ácaros do gênero Brevipalpus, e membro tentativo do gênero Cilevirus, teve sua primeira descrição em 1997 no estado de São Paulo, com potencial para causar perdas econômicas à cultura do maracujazeiro. Outro possível cilevirus, a leprose do ligustro, foi encontrado infectando Ligustrum spp., e possuía similaridade sintomatológica a um vírus descrito na Argentina em 1942, em Bella Vista, também presente em Ligustrum spp., e descrito como "lepra explosiva de la ligustrina". Porém, através da comparação de genomas o vírus encontrado no Brasil assemelhou- se com PfGSV, sendo possivelmente distinto do vírus encontrado na Argentina, sendo provável isolado de PfGSV, e assim designado como PfGSV-Lig. PfGSV-Lig foi encontrado infectando naturalmente várias espécies distintas de plantas, algumas das quais se infectaram experimentalmente, além de várias outras ensaiadas. A infecção foi comprovada através de ensaios de RT-PCR e exame de secções das lesões por microscopia eletrônica de transmissão. Ácaros tenuipalpídeos B. yothersi e B. papayensis haviam sido recentemente identificados como vetoras de PfGSV-Lig. Foram feitas tentativas de se obter colônias isolinhas destas espécies em folhas destacadas de algumas espécies de Ligustrum. Experimentos foram também realizados trabalhos para se determinar alguns parâmetros como período de acesso para aquisição e inoculação, e de latência do PfGSV-Lig, pelas espécies vetoras. Resultados preliminares de determinação do período de aquisição para inoculação indica ser ele menor que 24h. Estudos adicionais deverão definir estes parâmetros.
Title in English
Passion fruit green spot virus (PfGSV), isolated from Ligustrum: its relationships with the Brevipalpus vector mite
Keywords in English
Brevipalpus mite
PfGSV-Lig
Virus vector relationship
VTBs
Abstract in English
The passion fruit green spot virus (passion fruit green spot virus - PfGSV), transmitted by Brevipalpus mites, and tentative member of the genus Cilevirus, had its first description in 1997 in the state of São Paulo, with the potential to affect statistics to the passion fruit culture. Another possible virus, Ligustrum leprosy, has been found infecting Ligustrum spp. and it had symptomatic similarity to a virus described in Argentina in 1942, in Bella Vista, also present in the Ligustrum spp. It is described as "lepra explosiva de la ligustrina". However, through the comparison of genomes or viruses found in Brazil, associated with PfGSV, being possibly distinct from the virus found in Argentina, being probably isolated from PfGSV, and also as (PfGSV-Lig). PfGSV-Lig has been found to infect several different species of plants, some of which are infected experimentally, in addition to several others tested. The infection was proven through RT-PCR tests and examination of sections of lesions by transmission electron microscopy. Brevipalpus (Acari: Tenuipalpidae) mites present in PfGSV-Lig infected Ligustrum spp. plants were assayed to identify the vector species. B. yothersi transmitted PfGSV, but not B. obovatus and B. californicus. Experiments were also carried out to determine some parameters such as access period for acquisition and inoculation, and latency of PfGSV-Lig, by the vector species. Preliminary results for determining the acquisition period for inoculation indicate that it is less than 24 hours. Additional studies should define these parameters.
 
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Publishing Date
2020-05-06
 
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