• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Doctoral Thesis
DOI
https://doi.org/10.11606/T.10.2018.tde-13082018-111857
Document
Author
Full name
Maria Claudia Araripe Sucupira
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2003
Supervisor
Committee
Ortolani, Enrico Lippi (President)
Araujo, Wanderley Pereira de
Brito, Luiz Augusto Batista
Libera, Alice Maria Melville Paiva Della
Mattos, Wilson Roberto Soares
Title in Portuguese
Estudo comparativo de exames clínico-laboratoriais no diagnóstico de carência energética prolongada em garrotes
Keywords in Portuguese
Bovinos
Desnutrição protéico-energética
Ruminantes
Abstract in Portuguese
Para comparar indicadores no diagnostico laboratorial da carência energética e avaliar sua influencia no quadro clínico, 12 garrotes foram aleatoriamente distribuídos em três grupos iguais e receberam , por 140 dias, as seguintes dietas: (G1) adequada, para ganho de peso de 900g/dia (13% de PB e 17,7 Mcal/d de ED); (G2) 80% dos requerimentos de mantença (7% de PB e 5,8 Mcal/d de ED); e (G3) 60% desses requerimentos (5% de PB e 4,7 Mcal/d de ED). Dois animais do G3 sucumbiram ao término do experimento. A carência provocou menor consumo de alimentos, diminui9ao do escore de condição corporal, sem alterar o peso vivo. Embora o volume ruminal tenha sido semelhante entre todos os grupos, as taxa de passagem e de renovação de líquidos foram menores nos grupos carentes. O déficit energético reduziu a produção ruminal de ácidos graxos voláteis totais e de suas frações, o tempo de redução de azul de metileno no suco ruminal foi maior, enquanto que os índices de excreção urinaria de alantoína e de ácido úrico foram menores nos grupos carentes. Os melhores indicadores do status energético foram a glicemia e os teores de β-hidroxibutirato plasmáticos, que reduziram durante a carência: os AGLs não foram eficientes devido a alta variabilidade dos resultados. Não foram detectados corpos cetônicos na urina. O hematócrito e os teores séricos de ureia, albumina, globulinas e creatinina não se mostraram úteis no diagnóstico, porem o índice de excreção urinaria de ureia se reduziu, indicando maior conservação de nitrogênio pelo organismo carente. O déficit energético provocou diminui980 nos batimentos cardíacos, atingindo em quadros avançados 30 bat/min; na frequência respiratória; nos movimentos e tonicidade ruminal. Quanto menor foi a glicemia menores foram os batimentos cardíacos (r= 0,55), a frequência respiratória (r = 0,49) e a tonicidade ruminal (r= 0,81). A temperatura retal permaneceu dentro dos valores de normalidade, mas hipotermia foi detectada precedendo a morte. Levando-se em conta a facilidade, praticidade e custo dos exames, sugere-se o diagnóstico de carência requisitando analise de glicose, β-hidroxibutirato plasmáticos, tempo de redução do azul de metileno no suco ruminal e índice de excreção urinária de ácido úrico.
Title in English
Comparative study of clinical laboratory assays for the diagnosis of prolonged energetic deficit in steers
Keywords in English
Bovine
Proteic- energetic deficit
Ruminants
Abstract in English
In order to compare laboratory diagnosis indicators of energetic deficit and evaluate their influence in the clinical picture, 12 steers were randomly distributed in 3 groups of 4 animals, which received the following diets for 140 days : (G1) adequate diet for weight gain of 900g/day (CP: 13% and DE: 17,7 Mcal/d); (G2) 80% of the maintenance requirements (CP: 7% and DE: 5,8 Mcal/d); (G3) 60% of the same requirements (CP: 5% and DE: 4,7 Mcal/d). Two animals from G3 died by the end of the experiment. The nutritional deficit led to lower feed intake and a decrease in the body condition score; without changing body weight. Although the rumen volume was similar in all three groups, the rates of liquid passage and ruminal turnover were lower in G2 and G3. The energetic deficit led to a reduced rumen production of total and fractions of volatile fatty acids. The time of blue methylene reduction in the rumen juice was longer, whereas the index of urinary excretion of allantoin and uric acid was lower in G2 and G3. The best indicators of the energetic status were glycemia and the plasma level of β-hydroxybutirate, which were both reduced during the deficit energetic. NEFA levels were not effective, due to the high variability of the results. Ketone bodies were not detected in the urine. Hematocrit and serum levels of urea, albumin, globulin and creatinin were not useful for malnutrition diagnosis, but the index of urinary excretion of urea was decreased, indicating that malnourished bodies were keeping Nitrogen. The energetic deficit caused a gradual decrease in cardiac rates , falling to 30 beats/min in advanced deficit of energy. Reduced respiratory frequency and rumen tonus was also altered in this condition. Low glycemia was related to decreased heart beat frequency (r=0.55), to respiratory frequency (r=0.49) and to rumen tonus (r=0.81). The rectal temperature remained within normal values; however hypothermia was detected preceding death. Taking into account costs and friendly usage of assays, we suggest the analysis of glucose, plasma β-hydroxybutirate, time of reduction of methylene blue in the rumen juice, and index of urinary excretion of uric acid for the diagnosis of energetic deficit.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Publishing Date
2018-10-22
 
WARNING: Learn what derived works are clicking here.
All rights of the thesis/dissertation are from the authors
CeTI-SC/STI
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2024. All rights reserved.