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Master's Dissertation
DOI
https://doi.org/10.11606/D.10.2006.tde-02032007-155812
Document
Author
Full name
Adriana Tarlá Lorenzi
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Pirassununga, 2006
Supervisor
Committee
Martins, Maria de Fatima (President)
Faria, Dalva Lucia Araujo de
Merusse, Jose Luiz Bernardino
Title in Portuguese
Estudo colorimétrico e espectroscópico do muco de caracóis Achatina sp alimentados com rações acrescidas de plantas medicinais
Keywords in Portuguese
achatina fulica
escargots
espectroscopia infravermelho
espectroscopia Raman
muco
plantas medicinais
Abstract in Portuguese
Os caracóis terrestres pertencem às famílias Achatinidae (África) e Helicidae (Europa). A espécie mais conhecida é a Achatina fulica (Gigante africano), sendo a mais recomendada para as regiões tropicais e subtropicais devido a sua capacidade de adaptação a estes climas. O muco de caracóis terrestres Achatina sp tem sido pesquisado devido sua atividade cicatrizante, além da atividade antibacteriana. A suplementação (plantas com finalidades cicatrizantes definidas como: confrei, papaína e centelha asiática nas rações destes animais) teve o propósito de caracterizar a composição glicoproteica do muco, considerando o bem-estar do animal, haja vista que os mesmos não foram sacrificados. Fez-se uso de uma metodologia envolvendo a coleta através de estímulo manual da glândula podal, responsável pela secreção do muco. Metodologia esta divergente de alguns autores que fizeram uso de estímulo elétrico com corrente elétrica nos animais para a coleta do muco, método este que vai contra os propósitos de bem-estar animal. Este estudo, além de utilizar uma metodologia menos drástica e prejudicial aos animais, permitiu que os mesmos retornassem ao seu ambiente de criação. As análises realizadas por testes colorimétricos e espectroscópicos, constataram que as alterações apresentadas nos testes foram muito semelhantes; no entanto, mostraram uma variação significativa na composição glicoproteica dos mucos analisados.
Title in English
Colorimetric and spectroscopic study of mucus of Achatina sp snails fed with increased rations of medicinal plants
Keywords in English
achatina fulica
infrared spectroscopy
medicinals plants
mucus
Raman spectroscopy
scargots
Abstract in English
The terrestrial snails belong to the families Achatinidae (Africa) and Helicidae (Europe). The most known species is the Achatina fulica (Giant African), being the most recommended for the tropical and subtropical regions due its capacity of adaptation to these climates. Mucus of terrestrial snails Achatina sp has been searched had its wound healing activity, beyond the antibacterial activity. The suplementation (plants with defined wound healing purposes as comfrey, papain and centella asiatic in the rations of these animals) had the intention to characterize the glycoprotein composition of mucus, considering well-being of the animal, since the same ones had not been sacrificed. Using a methodology holding the collection through manual stimulation of the podal, responsible gland for the secretion of mucus. This methodology is divergent of some authors who had used electric stimulation with electric current on the animals for the collection of mucus, method contrary to the intentions of animal well-being. This study, beyond using a less drastic and harmful methodology to the animals, allowing that the same ones returned to its own creation environment. The analyses carried through for color and spectroscopy tests, evidenced that the alterations presented in the tests had been very similar; however, showed a significant variation in the glycoproteic composition of the analysed mucus.
 
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AdrianaTarlaLorenzi.pdf (758.62 Kbytes)
Publishing Date
2007-03-07
 
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