Diversidade genética de isolados de Mycobacterium bovis em rebanhos bovinos de diferentes localidades do Brasil

Souza Filho, Antonio Francisco de

doi:10.11606/T.10.2019.tde-25092019-145311

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Doctoral Thesis

DOI

https://doi.org/10.11606/T.10.2019.tde-25092019-145311

Document

Doctoral Thesis

Author

Souza Filho, Antonio Francisco de (Catálogo USP)

Full name

Antonio Francisco de Souza Filho

E-mail

Institute/School/College

Faculdade de Medicina Veterinária e Zootecnia

Knowledge Area

Experimental Epidemiology of Zoonosis

Date of Defense

2019-05-31

Published

São Paulo, 2019

Supervisor

Heinemann, Marcos Bryan (Catálogo USP)

Committee

Heinemann, Marcos Bryan (President)
Ferreira Neto, José Soares
Knöbl, Terezinha
Lage, Andrey Pereira
Ribeiro, Márcio Garcia

Title in Portuguese

Diversidade genética de isolados de Mycobacterium bovis em rebanhos bovinos de diferentes localidades do Brasil

Keywords in Portuguese

Spoligotyping
Tipagem
Tuberculose bovina
VNTR-MIRU

Abstract in Portuguese

A tuberculose bovina é uma doença causada pelo Mycobacterium bovis que afeta, principalmente, bovinos e búfalos. Para compreender melhor a distribuição da tuberculose bovina no país o presente trabalho teve como objetivo proceder a caracterização molecular de isolados de M. bovis de diferentes regiões do Brasil. No total 289 isolados provenientes de 11 estados brasileiros foram genotipados pelas técnicas de Spoligotyping e VNTR-MIRU com 24 marcadores diferentes, sendo o primeiro estudo com isolados de diferentes regiões do Brasil caracterizados por meio dessas técnicas. Foram identificados 42 espoligotipos e os perfis SB0121, SB0295, SB1380, SB0140 e SB1050 foram os mais frequentes, além disso três novos espoligotipos foram descritos. Por meio da técnica de VNTR-MIRU 173 perfis distintos foram caracterizados, mostrando alta diversidade desse agente no Brasil. Os marcadores com maior diversidade alélica foram: ETR-A, QUB11b, MIRU 16, MIRU 27, ETR-B, ETR-C, Mtub21 e QUB 26, sugerindo esse conjunto de marcadores capaz de caracterizar os isolados de M. bovis no Brasil.

Title in English

Genetic diversity of Mycobacterium bovis isolates in cattle herds from different regions of Brazil

Keywords in English

Bovine tuberculosis
Spoligotypin
Typing
VNTR-MIRU

Abstract in English

Bovine tuberculosis is a disease caused by Mycobacterium bovis that mainly affects cattle and buffalo. To better understand the distribution of bovine tuberculosis in the country, the present work aimed to characterize the molecular isolates of M. bovis from different regions of Brazil. In total, 289 isolates from 11 Brazilian states were genotyped by the Spoligotyping and VNTR-MIRU with 24 different markers, being the first study with isolates from different regions of Brazil characterized by these techniques. Forty-two spoligotypes were identified and the profiles SB0121, SB0295, SB1380, SB0140 and SB1050 were the most frequent, in addition three new spoligotypes were described. Through the VNTR-MIRU technique 173 distinct profiles were characterized, showing high diversity of this agent in Brazil. The markers with the highest allelic diversity were: ETR-A, QUB11b, MIRU 16, MIRU 27, ETR-B, ETR-C, Mtub21 and QUB 26, suggesting this set of markers capable of characterizing M. bovis isolates in Brazil.

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Publishing Date

2019-10-24

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