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Doctoral Thesis
DOI
https://doi.org/10.11606/T.10.2011.tde-25092012-110758
Document
Author
Full name
Cleise Ribeiro Gomes
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2011
Supervisor
Committee
Moreno, Andrea Micke (President)
Andrade, André Furugen Cesar de
Coutinho, Tania Alén
Knöbl, Terezinha
Zucon, Luciane Tieko Shinya
Title in Portuguese
Isolamento e caracterização genotípica de cepas de Bordetella avium através da eletroforese em campo pulsado (PFGE) e polimorfismo do comprimento de fragmentos amplificados (AFLP)
Keywords in Portuguese
Bordetella avium
AFLP
PCR
PFGE
Resistência antimicrobiana
Abstract in Portuguese
A Bordetella avium é o agente etiológico da bordetelose aviária, uma doença altamente contagiosa que afeta o trato respiratório superior das aves. B. avium adere-se preferencialmente às células do epitélio ciliado traqueal, promovendo inflamação e deformação da mucosa respiratória. As infecções do trato respiratório das aves resultam em grandes prejuízos para toda indústria avícola, desta forma, o presente estudo teve como objetivo a caracterização genotípica e de sensibilidade a antimicrobianos de isolados de B. avium provenientes de perus com histórico de aerossaculite. Dentre os 300 animais examinados, isolou-se B. avium de 13 aves e foram selecionadas 20 cepas do agente para os estudos posteriores. Através do antibiograma realizado pela técnica de disco difusão observou-se um alto número de cepas resistentes aos antimicrobianos beta lactâmicos (amoxacilina, ampicilina, penicilina e ceftiofur), assim como para lincomicina, sulfonamidas e combinação sulfonamidas/trimetoprima (cotrimoxazol) e uma grande heterogeneidade resultando em 15 perfis distintos. Os antimicrobianos com maiores níveis de sensibilidade foram o florfenicol, seguidos pelas quinolonas, doxiciclina e pelas tetraciclinas. Todas as cepas foram caracterizadas através da PFGE e do AFLP, apresentando 15 pulsotipos e 16 perfis genotípicos respectivamente. Os métodos fenotípicos e genotípicos apresentaram capacidade discriminatória semelhante e revelaram uma grande diversidade dentre os isolados analisados.
Title in English
Isolation and genotypic characterization of Bordetella avium strains by pulsed field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP)
Keywords in English
Bordetella avium
AFLP
Antimicrobial susceptibility
PFGE
Abstract in English
Bordetella avium is the etiologic agent of avian bordetellosis, a highly contagious disease that affects the upper respiratory tract of birds. B. avium adheres preferentially to ciliated tracheal epithelial cells, promoting inflammation and deformation of the respiratory mucosa. Infections of the respiratory tract of birds resulting in large losses for the entire poultry industry in this way, this study aimed to characterize genotypic and antimicrobial susceptibility of isolates of B. avium from turkeys with a history of Airsacculitis. Among the 300 animals examined, B. avium was isolated from 13 turkeys and 20 strains were selected for further studies. Through the antibiogram performed by disk diffusion technique was observed a high number of strains resistant to beta-lactamic antibiotics (amoxicillin, ampicillin, penicillin and ceftiofur), as well as, lincomycin, sulfonamides and sulfonamide combination/ trimethoprim (cotrimoxazole) and a high level of heterogeneity resulting in 15 different profiles. The antimicrobials with higher levels of sensitivity were florfenicol, followed by quinolones, doxycycline and tetracycline. All strains were characterized through to PFGE and AFLP, presenting 15 pulsotypes and 16 genetic profiles, respectively. Phenotypic and genotypic methods showed similar discriminatory capacity and presented a high diversity among isolates examined.
 
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Publishing Date
2013-04-15
 
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