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Master's Dissertation
DOI
https://doi.org/10.11606/D.10.2007.tde-25052007-130622
Document
Author
Full name
Cleise Ribeiro Gomes
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2006
Supervisor
Committee
Moreno, Andrea Micke (President)
Calderaro, Franco Ferraro
Ferreira, Antônio José Piantino
Title in Portuguese
Detecção de Lawsonia intracellularis em aves comerciais através da reação em cadeia pela polimerase (PCR)
Keywords in Portuguese
Lawsonia intracellularis
Aves
Histopatológico
Reação em cadeia pela polimerase
Abstract in Portuguese
A Lawsonia intracellularis é uma bactéria intracelular obrigatória que causa Enterite Proliferativa em vários animais, como suínos, cervos, ratos, hamsters, cobaios, coelhos, ovinos, eqüinos, raposas, cães, furões, primatas não humanos, emas e avestruzes. Atualmente não há relatos da ocorrência do agente ou dos sinais clínicos de sua infecção em aves comerciais (Gallus gallus domesticus). O presente estudo reporta a detecção através da reação em cadeia pela polimerase (PCR) de Lawsonia intracellularis em matrizes pesadas de diferentes idades provenientes de quatro linhagens de aves comerciais. Dentre os 100 suabes de fezes colhidos a partir de fragmentos intestinais, 34% foram positivos para a detecção do agente pela PCR. O agente foi encontrado com maior freqüência em aves de 80 semanas de idade apresentando ou não quadro diarréico. Os fragmentos intestinais das aves cujas fezes foram positivas para detecção de Lawsonia intracellularis foram submetidos ao exame histopatológico e na maioria das lesões analisadas foi observada enterite necrótica crônica proliferativa. Apesar das lesões serem sugestivas de infecção por Lawsonia intracellularis pela coloração de Warthin-Starry, a presença do patógeno no interior dos enterócitos ou células glandulares não foi observada. Esses resultados poderiam levar a novas pesquisas sobre a importância da Lawsonia intacellularis na avicultura do Brasil e do mundo.
Title in English
Detection of Lawsonia intracellularis in chicken by polymerase chain reaction (PCR)
Keywords in English
Lawsonia intracellularis
Chicken
Histophatological
Polymerase chain reaction
Abstract in English
Lawsonia intracellularis is an obligate intracellular bacterium responsible for proliferative enteritis in many animals, such as swine, deers, rats, hamsters, guinea pigs, rabbits, ovine, horses, foxes, dogs, ferrets, no human primats, emus and ostrichs. Currently there is no reports about the occurrence of this agent or clinical signs of its infection in chickens (Gallus gallus domesticus). The present study reports the detection of Lawsonia intracellularis at different ages of broiler breeder in four avian commercials lineages by Polymerase Chain Reaction (PCR). Of 100 faecal swabs collected from intestinal fragments, 34% were positive for Lawsonia intracellularis detection by PCR. The agent was more frequently found in chickens of 80 weeks of age presenting or not diarrhea. The intestinal fragments which faeces were positive for Lawsonia intracellularis PCR detection were submitted to histopathological exam and in the most analysed lesions were observed proliferative necrotic cronic enteritis. Although the lesions were suggestive of L. intracellularis infection by Warthin-Starry staining, the presence of the pathogen inside of enterocytes or gland cells was not observed. This results could lead to new researches about the mean of Lawsonia intacellularis in broiler industry of Brazil and the world.
 
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Publishing Date
2007-05-28
 
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