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Doctoral Thesis
DOI
https://doi.org/10.11606/T.10.2010.tde-24032011-152836
Document
Author
Full name
Tania Alén Coutinho
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Moreno, Andréa Micke (President)
Barcellos, David Emilio Santos Neves de
Guedes, Roberto Maurício Carvalho
Richtzenhain, Leonardo José
Timenetsky, Jorge
Title in Portuguese
Caracterização de amostras de Erysipelothrix spp. isoladas de suínos nos últimos 30 anos
Keywords in Portuguese
Erysipelothrix spp
AFLP
PFGE
Suínos
Susceptibilidade antimicrobiana
Abstract in Portuguese
Erysipelothrix rhusiopathiae é um importante patógeno em suinocultura e apesar do uso freqüente de vacinas contra o mesmo na maior parte das propriedades produtoras do país, a ocorrência de quadros clínicos da infecção tem sido amplamente observada e diagnosticada. Tendo em vista o ressurgimento deste agente como causa de prejuízos econômicos para a indústria suinícola nacional e seu potencial risco à saúde pública, este estudo teve como objetivo caracterizar 151 amostras de Erysipelothrix spp. isoladas de suínos nos útlimos 30 anos por meio de sorotipagem, determinação da susceptibilidade antimicrobiana, AFLP e PFGE. Dentre os 151 isolados, 139 foram classificados em 18 sorotipos diferentes (1a, 1b, 2a, 2b, 4, 5, 6, 7, 8, 10, 11, 12, 15, 17, 19, 21, 24 e 25), sendo que o sorotipo 2b foi o mais freqüente. Os perfis de susceptibilidade antimicrobiana foram muito semelhantes entre os isolados, o que impossibilitou a subtipagem dos isolados de Erysipelothrix spp. pelos testes de sensibilidade. Dentre os primers testados no AFLP, o HI-G foi o mais adequado à tipagem molecular de Erysipelothrix spp. Apesar do AFLP/HI-G e da PFGE apresentarem o mesmo índice discriminatório (0,98), a PFGE apresentou melhor relação com os dados epidemiológicos que o AFLP/HI-G, tendo em conta os agrupamentos por ela gerados. Independente da técnica molecular empregada, não foi observado a discriminação entre isolados recentes e históricos, bem como um padrão epidemiológico fixo de agrupamento dos mesmos. Contudo, o AFLP/HI-G pode ser uma alternativa interessante para diferenciar as espécies de Erysipelothrix, assim como a PFGE tem grande potencial para agrupar isolados deste gênero de acordo com os sorotipos.
Title in English
Characterization of Erysipelothrix spp. strains isolated from swine in last 30 years
Keywords in English
Erysipelothrix spp
AFLP
Antimicrobial susceptibility
PFGE
Swine
Abstract in English
Erysipelothrix rhusiopathiae is an important pathogen in swine production and even with the frequent use of vaccines against it in most of Brazilian pig farms, the occurrence of clinical manifestations of its infection has been widely observed and diagnosed. Given the resurgence of this agent as a cause of economic losses to the national pig industry and its potential risk to public health, this study aimed to characterize 151 samples of Erysipelothrix spp. isolated from swine in last 30 years by serotyping, determination of antimicrobial susceptibility, AFLP and PFGE. Among the 151 isolates, 139 were classified in 18 different serotypes (1a, 1b, 2a, 2b, 4, 5, 6, 7, 8, 10, 11, 12, 15, 17, 19, 21, 24 and 25) and serotype 2b was the most frequent. The susceptibility profiles were very similar among the isolates, which precluded the subtyping of Erysipelothrix spp. isolates by sensitivity tests. Among the primers tested in AFLP, the HI-G was the most suitable for molecular typing of Erysipelothrix spp. Despite AFLP/HI-G and PFGE provided the same discriminatory index (0.98), PFGE presented better relationship with epidemiological data than AFLP/HI-G, given the types of groups generated by it. Regardless of the molecular technique employed, there was no discrimination between recent and historical isolates as well as a fixed epidemiological pattern of grouping them. Nevertheless AFLP/HI-G could be an interesting alternative for Erysipelothrix species discrimination, even as PFGE has a good potential to diferenciate this genus according to serotypes.
 
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Publishing Date
2011-04-14
 
WARNING: The material described below relates to works resulting from this thesis or dissertation. The contents of these works are the author's responsibility.
  • COUTINHO, Tania A., et al. Phenotypic and molecular characterization of recent and archived Erysipelothrix spp. isolated from Brazilian swine [doi:10.1016/j.diagmicrobio.2010.09.012]. Diagnostic Microbiology and Infectious Disease [online], 2011, vol. 69, n. 2, p. 123-129.
  • COUTINHO, Tania Alen, et al. Characterization of Erysipelothrix rhusiopathiae isolated from Brazilian Tayassu pecari [doi:10.1007/s11250-011-9980-4]. Tropical Animal Health and Production [online], .
  • COUTINHO, Tania Alen, et al. Genotyping of Brazilian Erysipelothrix spp. strains by amplified fragment length polymorphism [doi:10.1016/j.mimet.2010.10.003]. Journal of Microbiological Methods [online], 2011, vol. 84, n. 1, p. 27-32.
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