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Doctoral Thesis
DOI
https://doi.org/10.11606/T.10.2016.tde-16092015-093411
Document
Author
Full name
Marina Moreno
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2015
Supervisor
Committee
Ferreira Neto, José Soares (President)
Andrade, André Furugen Cesar de
Calderaro, Franco Ferraro
Knöbl, Terezinha
Oliveira, Luís Guilherme de
Title in Portuguese
Caracterização genotípica e fenotípica de cepas de Escherichia coli associadas à diarreia pós-desmame em suínos
Keywords in Portuguese
Escherichia coli
Antimicrobianos
Diarreia
Pós-desmame
Resistência
Suínos
Abstract in Portuguese
A Escherichia coli é um dos principais agentes envolvidos na síndrome da diarreia pós-desmame em suínos, sendo a infecção de difícil controle devido à elevada capacidade deste agente em desenvolver e disseminar mecanismos de resistência aos antimicrobianos utilizados para o tratamento da doença. No presente estudo foi estabelecido o perfil de virulência de cepas de E. coli isoladas de 621 leitões com sintomas de diarreia na fase de creche (21 a 40 dias de vida), provenientes de 52 granjas de suínos no Brasil. Foram selecionadas 148 cepas de E. coli, positivas para fímbria F4 e /ou F18 e destas foi determinado o perfil de resistência à antimicrobianos por determinação da concentração inibitória mínima para os seguintes antimicrobianos: ampicilina, clindamicina, clortetraciclina, danofloxacina, enrofloxacina, florfenicol, gentamicina, neomicina, oxitetraciclina, sulfadimetoxina, espectinomicina, trimetoprima / sulfametoxazole, tiamulina, tilmicosina, tulatromicina, tilosina, ceftiofur e enrofloxacina. Dos 621 animais observados, 321 animais foram positivos para pelo menos um fator de virulência. Das cepas estudadas, todas as 148 foram resistentes a tilosina, clindamicina, tiamulina, tilmicosina e sulfadimetoxina. A frequência de cepas resistentes foi elevada também para o oxitetraciclina, clortetraciclina, danofloxacina, ampicilina e ao florfenicol. Considerando-se o fato de 100% das cepas terem apresentado resistência a mais de três classes de antimicrobianos pode-se concluir que todas as cepas de E.coli do presente estudo foram multirresistentes sendo frequente a resistência aos principais antimicrobianos utilizados na terapia da diarreia em leitões de creche
Title in English
Genotypic and phenotypic characterization of Escherichia coli strains associated with from post-weaning swine
Keywords in English
Escherichia coli
Antimicrobials
Diarrhea
Post-weaning
Resistance
Swine
Abstract in English
Escherichia coli is one of the agents involved in the syndrome of post-weaning diarrhea in swine. The infection is difficult to control because of high ability of this agent to develop and spread antimicrobial resistance mechanisms against commonly used antibiotics. This study has as goal determine the virulence profile of E. coli strains isolated of 621 piglets with diarrhea symptoms in the nursery with 21-40 days of age, from 52 pig farms in Brazil. Of the isolated strains, we selected 148 strains of E. coli, positive for F4 fimbriae and / or F18 and these were tested for antimicrobial resistance for determining the minimum inhibitory concentration for the following antibiotics: ampicillin, clindamycin, chlortetracycline, danofloxacin, enrofloxacin, florfenicol, gentamicin, neomycin, oxytetracycline, sulfadimethoxin, spectinomycin, trimethoprim / sulfamethoxazole, tiamulin, tilmicosin, tulathromycin, tylosin, ceftiofur and enrofloxacin. Of the 621 observed animals 321 animals were positive for at least one virulence factor. Of the studied strains, all 148 were resistant to tylosin, clindamycin, tiamulin, tilmicosin and sulfadimethoxin. The frequency of resistant strains was also high for oxytetracycline, chlortetracycline, danofloxacin, ampicillin and florfenicol. Considering the fact that 100% of the strains have shown resistance to more than three classes of antimicrobials can be concluded that all E. coli strains in this study were multidrug-resistant and are frequent the resistance to the main antimicrobials used in therapy of diarrhea in nursery pigs
 
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Publishing Date
2016-04-08
 
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