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Doctoral Thesis
DOI
https://doi.org/10.11606/T.10.2013.tde-04062013-112008
Document
Author
Full name
Sibele Pinheiro de Souza
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2013
Supervisor
Committee
Brandão, Paulo Eduardo (President)
Jerez, José Antonio
Kawai, Juliana Galera Castilho
Sakamoto, Sidnei Miyoshi
Salgado, Vanessa Riesz
Title in Portuguese
Um estudo sobre a diversidade molecular dos genes S e HE de Coronavírus bovino (BCoV)
Keywords in Portuguese
Coranavírus Bovino (BCoV)
Diversidade
Gene HE (hemaglutinina - esterase)
Gene S (espícula)
Abstract in Portuguese
Coronavírus bovino (BCoV) é o agente causador de doença, tanto entérica como respiratória em bovinos, mas até agora existem controvérsias sobre a relação genealógica entre as amostras de BCoV em diferentes tecidos. Neste estudo, amostras de fezes e secreções nasais de 14 vacas de um mesmo rebanho apresentando simultaneamente disenteria epizoótica e doença respiratória foram estudados quanto a presença de BCoV. As amostras virais detectadas tiveram tanto o gene de espícula (S) como o gene hemaglutinina-esterase (HE) parcialmente sequenciados. Para o gene HE, foram obtidas 12 sequências de secreções nasais e 12 de amostras de fezes e para o gene S, foram obtidas 14 sequências de secreções nasais e 12 de amostras de fezes, com 100% de identidade nucleotídica para cada gene para as amostras deste estudo. Estes resultados apresentam algumas divergências com estudos anteriores os quais relatam que linhagens diferentes de BCoV podem ser esperados em casos de disenteria e doença respiratória em vacas, pois linhagens com sequências idênticas dos genes S e HE podem não mostrar diferenças em relação tropismo pelos diferentes tecidos. Sequências completas de duas amostras brasileiras de BCoV mostram que o já descrito padrão filogeográfico baseado no sequenciamento do gene S parcial foi mantido, foram encontradas substituições de aminoácidos específicos.
Title in English
A study on the molecular diversity of S and HE genes of Bovine coronavirus (BCoV)
Keywords in English
Bovine coranavirus (BCoV)
Diversity
HE Gene (hemagglutinin - esterase)
S Gene (spike)
Abstract in English
Bovine coronavirus (BCoV) is the causative agent of both enteric and respiratory disease in cattle, but hitherto there were some controversy on the genealogic relationship amongst strains from these different tissues. In this study, samples of feces and nasal secretions of 14 cows from a same herd simultaneously presenting epizootic dysentery and respiratory disease were screened for BCoV and the strains detected had both the spike (S) and hemagglutinin-esterase (HE) genes partially sequenced. For HE gene, 12 sequences from nasal secretions and 12 from fecal samples were obtained and for S gene, 14 sequences from nasal secretions and 12 from fecal samples were obtained, with 100% nucleotide identities for each gene for the strains of this study. These results have some disagreements with previous reports which try to put forward that divergent BCoV strain should be expected in cases of dysentery and respiratory disease in cows, showing that strain with identical S and HE sequences might show no differences in tropisms. Complete S gene sequences of two Brazilian BCoV strains show that the already described phylogeographic pattern based on partial S gene is sustained, though specific amino acids subtitutions are found.
 
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Publishing Date
2013-09-18
 
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