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Master's Dissertation
DOI
https://doi.org/10.11606/D.10.2003.tde-29042004-180938
Document
Author
Full name
Gustavo Coelho Rodrigues
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2003
Supervisor
Committee
Kfoury Junior, Jose Roberto (President)
Meirelles, Flavio Vieira
Sinhorini, Idercio Luiz
Title in Portuguese
Caracterização ultraestrutural de células do sangue de cordão umbilical de bovinos azebuados
Keywords in Portuguese
bovinos
cordão umbilical
microscopia eletrônica
sangue
ultraestrutura
Abstract in Portuguese
Estudos envolvendo a utilização do sangue de cordão umbilical foram intensificados na última década, devido ao grande potencial que estas possuem nas pesquisas de transplantes e ontogenia celular. A investigação dos métodos para a purificação e caracterização dessas células em diferentes animais pode aumentar a utilização destes como modelos experimentais para uma variedade de propostas científicas e terapêuticas. Para desenvolver a caracterização das células de sangue de cordão umbilical foram utilizadas 20 amostras oriundas de fetos de com idades compatíveis ao segundo e terceiro terço de gestação. Estas amostras foram processadas de duas formas distintas. A primeira forma visou às células presentes na camada leucocitária total, a segunda visou as células com densidade menor que 1077 separadas pelo Ficoll Paque. Os “pellets" resultantes foram processados para posterior observação por microscopia eletrônica de transmissão. Foram constatadas a presença de células granulares (basófilos, eosinófilos e neutrófilos) e agranulares (células bláticas, precursores eosinofílicos e linfócitos) em diferentes estágios de maturação em ambos os grupos e também células com características morfológicas compatíveis a apoptose.
Title in English
Ultraestructural characterization of umbilical cord blood of bovine
Keywords in English
animal umbilical cord
blood
bovine
eletronic microscopic
ultraestructural
Abstract in English
Studies involving the use of umbilical cord blood were intensified in the last decade, due to its huge potential for transplant research and the ontogeny of leucocytes cells. The investigation of the methods to purify and characterize these cells in different animals are a base for experimental models for a variety of scientific and therapeutic proposals. To develop the characterization of the umbilical cord cells, twenty samples of fetal were collected. The fetal were in the second and third thirds of the gestation. These samples were processed in two distinct forms. The first form, studied the cells in the buffet coat. The second, studied the cells with density less than, 1,077 separated by the Ficoll Paque. The resulting pellets were processed for later observation by electronic microscopy transmission. The presence of granular and non granular cells were detected in different maturation stages in both groups and also morphological compatible cells with apoptosis.
 
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gustavocoelho.pdf (2.81 Mbytes)
Publishing Date
2004-09-14
 
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