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Master's Dissertation
DOI
https://doi.org/10.11606/D.10.2007.tde-14022008-163944
Document
Author
Full name
João Carlos Morini Junior
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2007
Supervisor
Committee
Bombonato, Pedro Primo (President)
Pereira, Flávia Thomaz Veréchia
Ribeiro, Luiz Alberto Oliveira
Title in Portuguese
Glicogênio placentário e fetal originados de ovinos gestantes submetidos ou não a tosquia
Keywords in Portuguese
Estresse
Ovelhas
Placenta
Relação materno-fetal
Tosquia
Abstract in Portuguese
Com intuito de esclarecer a associação do ganho de peso do feto no ato da tosquia, fato este, que diminui a taxa de mortalidade ao nascer, utilizamos vinte e uma ovelhas da raça merino australiano, divididos em 2 grupos com 10 integrantes cada: um tosquiado (OT) aos 70 dias do período gestacional e um segundo mantido como controle (ONT). Destas fêmeas durante o período final de gestação retirou-se a placenta e o feto através do procedimento cirúrgico segundo técnica cesariana convencional. Para efeito comparativo mensurou-se inicialmente as características morfométricas da placenta e dos fetos. Como caracterização da placenta quantificamos o número de placentônios e suas medidas. As amostras de placenta foram fixadas e processadas para microscopia de luz, com inclusão em parafina. A quantificação do glicogênio foi obtida com a mensuração de 5 campos randômicos de cada lâmina produzida seqüencialmente. As análises foram realizadas por efeito comparativo de diferença de coloração por reação histoquímica de P.A.S. Para análise estatística foi utilizado o teste T normal comparativo entre médias. Os resultados macroscópicos indicaram que o tratamento da tosquia não aumentou significativamente os pesos placentários e fetais, e tão pouco as medidas referentes ao placentônio. Nas análises microscópicas, a quantidade média de glicogênio entre as áreas quantificadas no placentônio não apresentaram diferenças significativas, já no fígado na região da tríade portal e no músculo reto houve diferença entre os animais tosquiados e os não tosquiados. Com isto podemos concluir que a tosquia realizada aos 70 dias do período gestacional influência no acumulo de glicogênio no fígado e no músculo reto femural, podendo ser estes os fatos responsáveis pelo aumento do peso dos fetos ao nascer.
Title in English
Glycogen related to placenta and fetus of shorned or not shorned sheep during the gestation
Keywords in English
Lambs
Materno-fetal relationship
Placenta
Shear
Stress
Abstract in English
In order to clarify the association of weight increase of the fetus in the act of shorn, and remembered that this fact decreases the rate of mortality at birth we tested twenty Merino australian sheep. Animals were divided into 2 groups with 10 members each: shorn (OT) to 70 days of gestational period and a second kept as control (ONT). The placenta and fetus were removed by a surgical procedure of caesarean, according conventional technique. As characterization of the placenta quantification of the placentome numbers and their measures. Samples of placenta were fixed and routinely processed for the light microscopy, with inclusion in paraffin. The quantification of glycogen was obtained with the measurement of 5 fields in each slides produced sequentially. The tests were conducted by comparative effect of a difference in color between the slides and the area covered by the histochemistry PAS reaction For statistical analysis was used the T test (student) and comparison between normal averages. The macroscopic results indicated that the treatment of shorn not significantly increased the fetal and placental weights, and so little measures regarding placentomes. The microscopic analysis, shows that the average between the areas of glycogen quantified in placentome were not different (P <0.05). Already in the liver at the portal triad region and in the rectus femoris muscle were found a significant increase of glycogen deposits (P <0.05). With this results we can conclude that the shorn done at 70 days of gestational period influence on the accumulation of glycogen in the liver and muscle of the fetuses and thus increase the weight of them at birth.
 
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Publishing Date
2008-03-07
 
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