http://www.teses.usp.br Últimas 10 publicaciones de la Biblioteca Digital de Tesis y Disertaciones de la Universidad de São Paulo Biomedical Sciences Institute es Fri, 24 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/42/42133/tde-19042013-092628/?&lang=es http://www.teses.usp.br/teses/disponiveis/42/42133/tde-19042013-092628/?&lang=es Memory T cells are the hallmark of adaptive immunity and are characterized as central (TCM) and effector memory (TEM) T cells. The influence of T cells in the course of hematological malignancies has been described as a mechanism related to the evolution. In this study, we analyzed the peripheral blood of healthy donors and patients with myelodysplastic syndrome (MDS), multiple myeloma (MM) and chronic lymphocytic leukemia B (CLL), and analyzed the distribution of CD4+ and CD8+ naive and memory T... tde@sc.usp.br (Correia, Rodolfo Patussi) Disertación de Máster Fri, 24 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/42/42133/tde-19042013-091132/?&lang=es http://www.teses.usp.br/teses/disponiveis/42/42133/tde-19042013-091132/?&lang=es Dendritic cells (DCs) are the main antigen presenting cells (APCs). It was proposed that DCs select the Ags that will be presented at their surfaces on class II based on the presence of a TLR-ligand on the cargo. However, the relevance of the coupling of the Ag to a TLR-ligand on class I was not yet addressed. Thus, the goal of this study was to investigate whether the coupling of particulate Ags to a TLR-agonist improve the cross-presentation (CP). We saw that the CP does not involve endosomal acidification... tde@sc.usp.br (Baleeiro, Renato Brito) Tesis Doctoral Thu, 23 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/42/42133/tde-19042013-085322/?&lang=es http://www.teses.usp.br/teses/disponiveis/42/42133/tde-19042013-085322/?&lang=es Malaria is responsible to kill over a million people a year worldwide. The complications of the disease in humans infected by parasites of the genus Plasmodium, appears to be to the exacerbated immune response to infection. Inhibitory molecules such as PD-1 and PD-L1 are expressed at high levels in T cells in mice in the acute phase of infection by P. chabaudi. Then, the aim was to examine the role of PD-1 and PD-L1 in the activation and regulation of T spleen cells of mice during acute infection... tde@sc.usp.br (Severi, Letícia Sarturi Pereira) Disertación de Máster Thu, 23 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/42/42133/tde-19042013-083444/?&lang=es http://www.teses.usp.br/teses/disponiveis/42/42133/tde-19042013-083444/?&lang=es Experimental data in rodents indicate that maternal allergen-specific IgG prevents allergic sensitization in the progeny. Airborne allergens in maternal milk also protects breastfeed mice by oral tolerance induction. In this study, we assessed in humans whether Der p and Blo t allergens and specific antibodies were transferred during pregnancy and breastfeeding in a cohort of 91 paired samples of maternal blood, colostrums and umbilical cord blood. Our study indicates that Der p 1 and Blo t 5 can... tde@sc.usp.br (Macchiaverni, Patrícia) Tesis Doctoral Thu, 23 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/42/42133/tde-18042013-104537/?&lang=es http://www.teses.usp.br/teses/disponiveis/42/42133/tde-18042013-104537/?&lang=es We investigated the soluble guanylate cyclase agonist, BAY 41-2272, as an alternative to compensate for failures in of monocytes function. We evaluated the in vitro effect of the drug on human cells lines and peripheral blood cells. The BAY increased expression of CD11b, CD18, CD14, TLR4, TLR2 and CD163 and induce the production of TNF-a, IL-1b, IL-6 and IL-12p70 in THP-1 cells and monocytes. Furthermore, the drug increased NADPH oxidase gene (CYBB, CYBA and NCF2) and protein (gp91phox and p67phox)... tde@sc.usp.br (Pereira, Paulo Vitor Soeiro) Tesis Doctoral Thu, 23 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/42/42133/tde-18042013-102717/?&lang=es http://www.teses.usp.br/teses/disponiveis/42/42133/tde-18042013-102717/?&lang=es Mice AIRmax and AIRmin differ on sensibility to carcinogenesis. AIRmin mice are significantly more sensitive to skin carcinogenesis than AIRmax mice due to the genetic background and the polymorphism of aryl hydrocarbon receptor (Ahr) gene. Furthermore,these mice have an imbalance of frequency of Slc11a1 gene alleles.To study the interaction of resistant (R) or susceptible (S) Slc11a1 alleles with acute inflammatory reaction loci found in AIRmax and AIRmin mice, homozygous sublines for these alleles... tde@sc.usp.br (Paula, Mariana Perlati Santos Aleixo de) Disertación de Máster Thu, 23 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/42/42133/tde-18042013-094555/?&lang=es http://www.teses.usp.br/teses/disponiveis/42/42133/tde-18042013-094555/?&lang=es We identified 12 CD40L-deficient patients with an incidence of fungal infections of 83%, including the first case of paracoccidioidomycosis and aspergillosis. The immature DCs (iDCs) from the patients present reduced protein expression of HLA-DR, CD80 and CD86 and mature DCs (mDCs) pulsed with P. brasiliensis (mDCs) presented low expression of HLA-DR and CD80 molecules The mDCs from the patients secreted low levels of IL-12 and high of IL-10. In co-cultures of mDCs and autologous T lymphocytes we... tde@sc.usp.br (Marques, Otávio Cabral) Tesis Doctoral Thu, 23 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/42/42136/tde-18042013-093501/?&lang=es http://www.teses.usp.br/teses/disponiveis/42/42136/tde-18042013-093501/?&lang=es Protein disulfide isomerase (PDI) was identified as a possible regulator of NADPH oxidase (Nox) vascular, major enzyme responsible for the reactive oxygen species (ROS) production in hypertension. The aim of this study was to investigate the role of PDI in ROS generation in spontaneously hypertensive rats (SHR) vascular beds during hypertension development. The results showed an increase in ROS generation, Nox1 and PDI expression in mesenteric, coronary and pulmonary arteries from SHR with 8 and... tde@sc.usp.br (Androwiki, Aline Cristianne Depoli) Disertación de Máster Thu, 23 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/42/42136/tde-18042013-085640/?&lang=es http://www.teses.usp.br/teses/disponiveis/42/42136/tde-18042013-085640/?&lang=es Crotoxin (CTX), the major toxin of Crotalus durissus terrificus venom induces an inhibitory effect on tumoral growth and modulates, particularly, the functions of macrophages, fundamental cells to provide a defense mechanism against tumor cell. In early tumor progression, macrophages are avidly phagocytic (inflammatory cell) releasing reactive nitrogen intermediates-RNI/ROI and cytokines TNF-a, IL-1b and IL-6. However, when the tumor is installed, tumor-associated macrophage (angiogenic cell) presents... tde@sc.usp.br (Faiad, Odair Jorge) Disertación de Máster Wed, 22 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/42/42136/tde-18042013-083733/?&lang=es http://www.teses.usp.br/teses/disponiveis/42/42136/tde-18042013-083733/?&lang=es The cardiac remodeling is a sequel of hypertension. Toll-Like Receptors (TLR) are innate immunity receptor and its activation produces inflammatory molecules. The objective of this study was to evaluate the involvement of TLR4 in cardiac remodeling of spontaneously hypertensive rats (SHR). We used male Wistar and SHR with 6 and 21 weeks. The level of TLR4 mRNA in SHR with 21 weeks is higher than the other groups. Wistar and SHR with 19 weeks were treated with anti-TLR4 or nonspecific IgG antibody... tde@sc.usp.br (Pereira, Cinthya Echem de Souza) Disertación de Máster Wed, 22 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/42/42134/tde-17042013-114031/?&lang=es http://www.teses.usp.br/teses/disponiveis/42/42134/tde-17042013-114031/?&lang=es Cancer Cachexia, whose most notable symptom is severe and rapid weight loss, affects about half of all cancer patients and is present in the vast majority (more than two thirds) of patients with advanced cancer. Recent studies have shown that inflammatory mediators play an important role in the development of cachexia, nowadays envisaged as a chronic inflammatory disease. The white adipose tissue is one of the first compartments of the organism affected in cancer cachexia, and suffers a high rate... tde@sc.usp.br (Camargo, Rodolfo Gonzalez) Disertación de Máster Wed, 22 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/42/42134/tde-17042013-113528/?&lang=es http://www.teses.usp.br/teses/disponiveis/42/42134/tde-17042013-113528/?&lang=es In the present thesis we analyzed the involvement of E3 ligases under three aspects of skeletal muscle plasticity: mass loss resulting from disuse, maintenance of fiber type I and II and regeneration of muscle tissue. Our first aim was to determine whether leucine, was able to attenuate the mass loss caused by wasting. Our results showed that this amino acid prevented the mass loss, mainly by inhibiting the expression of E3 ligases. The second aim was to determine whether the absence of two E3 ligases,... tde@sc.usp.br (Baptista, Igor Luchini) Tesis Doctoral Wed, 22 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/42/42134/tde-17042013-105432/?&lang=es http://www.teses.usp.br/teses/disponiveis/42/42134/tde-17042013-105432/?&lang=es Gliomas are tumors of the central nervous system (CNS) that evolve from glial cells. The most common and most aggressive form of these tumors is known as glioblastoma multiforme (GBM). The biological aggressiveness of GBM is associated with its rapid growth and lack of apoptosis. Its poor prognosis is strongly associated with the difficulty of treating these cells as they are resistant to chemotherapy and radiotherapy. The gene expression of the enzymes cyclooxygenase-1 (COX-1), cyclooxygenase-2... tde@sc.usp.br (Cunha, Andrew Silva da) Disertación de Máster Wed, 22 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/42/42137/tde-17042013-105052/?&lang=es http://www.teses.usp.br/teses/disponiveis/42/42137/tde-17042013-105052/?&lang=es The current work examined the possible contribution of signal to noise ratio, the asynchrony between the onsets of the cue and the target (SOA) and the kind of task performed by the observer to the manifestation of crossmodal attentional effects. The Experiments 1 and 2 showed that crossmodal attentional effect appears when there is visual noise, but it does not appear when there is no visual noise at 133 ms SOA. The Experiment 3 showed that when the SOA is longer than 133 ms, the crossmodal attentional... tde@sc.usp.br (Righi, Luana Lira) Tesis Doctoral Wed, 22 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/42/42137/tde-17042013-101853/?&lang=es http://www.teses.usp.br/teses/disponiveis/42/42137/tde-17042013-101853/?&lang=es GLUT4 (gene Slc2a4) is responsible by insulin-induced glucose uptake and alterations in its expression are related to insulin resistance (IR). Variability in estradiol levels (E2) is related with IR and lower glucose transporter expression and this mechanism can be mediated by transcriptional factor NFk-B, which is an Slc2a4 repressor. Our aim was to evaluate in 3T3-L1 adipocytes the role of E2 in Slc2a4/GLUT4 expression, NFk-B binding activity and glucose uptake as well as the ESR1 (estrogen receptor... tde@sc.usp.br (Campello, Raquel Saldanha) Tesis Doctoral Wed, 22 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/42/42137/tde-17042013-095626/?&lang=es http://www.teses.usp.br/teses/disponiveis/42/42137/tde-17042013-095626/?&lang=es Factors such as age, sleep and the ability to engage attention to time contribute to the subjective measure of the time flow. We aimed to describe the daily modulation in the production of time as a function of aging and to determine associations between temporal profiles of this behavior and the circadian rhythm of peripheral temperature and sleep/wake cycle. Our findings: (1) the prevalence of social Jet-lag in young adults; (2) a distinct arrangement in the internal temporal organization for each... tde@sc.usp.br (Miguel, Mario André Leocadio) Tesis Doctoral Tue, 21 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/42/42137/tde-17042013-095205/?&lang=es http://www.teses.usp.br/teses/disponiveis/42/42137/tde-17042013-095205/?&lang=es We sought to identify the cellular effects by which DHEA improves pancreatic islets function. Male Wistar rats, 12-14 month-old, receive one subcutaneous injection of DHEA (10mg.kg-1) or vehicle. After a week, pancreatic islets from these animals were isolated and submitted to the following tests: insulin secretion, mitochondrial respiration, glucose oxidation, citrate synthase activity, DNA fragmentation, gene expression and the dosage of plasmatic steroid hormones. DHEA treatment resulted in significant... tde@sc.usp.br (Almeida, Felipe Natali) Tesis Doctoral Tue, 21 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/42/42131/tde-17042013-093520/?&lang=es http://www.teses.usp.br/teses/disponiveis/42/42131/tde-17042013-093520/?&lang=es Stimulation of the motor cortex (MC) has been used to treat patients with pain syndromes resistant to other treatments. Data from our group demonstrates that electrical stimulation of the motor cortex (MCS) induces opioid-dependent analgesia in rats. This study investigated the topography of the antinociceptive effect induced by MCS and the pattern of neuronal activation in the dorsal horn of the spinal cord (DHSC) and in the PAG through Fos and Egr-1 expression. Rats received implantation of transdural... tde@sc.usp.br (França, Nubia Regina Moreira) Disertación de Máster Tue, 21 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/42/42131/tde-17042013-085753/?&lang=es http://www.teses.usp.br/teses/disponiveis/42/42131/tde-17042013-085753/?&lang=es Heat shock proteins play a key regulatory role in cellular defense. In order to investigate the role of the inducible 70-kDa heat shock protein (HSP70) in skeletal muscle atrophy and subsequent recovery, extensor digitorum longus and soleus muscles from overexpressing HSP70 transgenic mice were immobilized during 7 days and subsequently released from immobilization and evaluated after 7 days. There was a decrease in myofiber cross-sectional area after immobilization in both wild type and HSP70 mice,... tde@sc.usp.br (Nascimento, Tábata Leal) Disertación de Máster Tue, 21 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/42/42131/tde-17042013-084156/?&lang=es http://www.teses.usp.br/teses/disponiveis/42/42131/tde-17042013-084156/?&lang=es The proliferation of adrenal cortical cells involves genes and proteins related with the control of the cell cycle. The main factor involved in the proliferation of the adrenal cortex is the ACTH (corticotrophin), but other factor, such as the peptide N-terminal of POMC, seems to be involved. To contribute to the understanding of the mechanisms involved in the proliferative response of the adrenal cortex, we analyzed the effects triggered by both peptides in rats with the HPA axis inhibited by dexamethasone.... tde@sc.usp.br (Mendonça, Pedro Omori Ribeiro de) Tesis Doctoral