http://www.teses.usp.br Last 10 publications of the Digital Library of Theses and Dissertations of University of São Paulo Biotechnology en Thu, 02 May 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/87/87131/tde-26112012-092054/?&lang=en http://www.teses.usp.br/teses/disponiveis/87/87131/tde-26112012-092054/?&lang=en The Chronic Obstructive Pulmonary Disease (COPD) stands out as a serious health problem in the world. The pulmonary emphysema, in the spectrum of COPD, has as main feature the enlargement of the airspaces distal resulting from destruction of lung parenchyma. Smoking is considered the main factor related to the development of pathology. Considering the lack of machines marketed for exposure to cigarette smoke, as well as the high cost of devices available, the currently project has proposed a experimental... tde@sc.usp.br (Kozma, Rodrigo de Las Heras) Master's Dissertation Thu, 21 Mar 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/87/87131/tde-21032013-170410/?&lang=en http://www.teses.usp.br/teses/disponiveis/87/87131/tde-21032013-170410/?&lang=en Breast cancer is one of the most commun tumors in women. Some populations shows higher risks for breast and ovarian cancers due to founder mutations in the BRCA1 and BRCA2 genes, as well as Ashkenazi Jewish (AJ) population. Genetic tests to detect point mutations in the BRCA1 and BRCA2 genes were made in 106 individuals: Ashkenazi Jewish and non-Ashkenazi Jews (NAJ) using dHPLC screening and sequencing. The distribution of founder mutations in the BRCA1 and BRCA2. genes was about 55 % for 185delAG... tde@sc.usp.br (Cunha, Danielle Renzoni da) Doctoral Thesis Fri, 22 Feb 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/87/87131/tde-05022013-090052/?&lang=en http://www.teses.usp.br/teses/disponiveis/87/87131/tde-05022013-090052/?&lang=en This study aimed to evaluate the immunomodulatory effects of immature stem cells from dental pulp on differentiation, maturation of monocyte-derived dendritic cells (mo-DC), their ability to activate T cells, as well as identify soluble factors released in cellular cocultures. The analyzes were performed by flow cytometry. It was observed a decrease of molecules related to mo-DC differentiation and an increase of molecules related to precursor state, which seems to have reflected to mDC maturation... tde@sc.usp.br (Silva, Fernando de Sá) Doctoral Thesis Fri, 22 Feb 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/87/87131/tde-20022013-154151/?&lang=en http://www.teses.usp.br/teses/disponiveis/87/87131/tde-20022013-154151/?&lang=en The proteins E7 (HPV), p24 (HIV) and gD (HSV) are exclusively expressed by infected cells or tumors and therefore are used as targets for vaccines with therapeutic characteristics. We developed two DNA vaccines capable of expressing these three viral proteins using a bicistronic expression vector based on IRES sequence. The plasmid vaccines, named pIRES I and pIRES II, differ by carrying the genes that encode proteins of HPV-16 E7 and p24 fused to the HIV protein gD of HSV-1 in reverse order. Transfected... tde@sc.usp.br (Santana, Vinicius Canato) Master's Dissertation Fri, 22 Feb 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/87/87131/tde-05022013-093824/?&lang=en http://www.teses.usp.br/teses/disponiveis/87/87131/tde-05022013-093824/?&lang=en Cervical cancer is the third most common cancer women worldwide and HPV16 infection is a major risk factor for its ethiopathogenesis. In this study we evaluated in mice the prophylactic and therapeutic potential of the E6E7 proteic vaccine, consisting of immunogenic HPV16 E6 and E7 epitopes, with or without Ubiquitin-fused (E6E7Ub and UbE6E7), against TC-1 tumor cells. The E6E7 vaccine conferred complete protection against tumor growth in previously immunized wild-type, CD4 knockout and TLR4 deficient... tde@sc.usp.br (Oliveira, Liliane Maria Fernandes de) Master's Dissertation Fri, 22 Feb 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/87/87131/tde-05022013-092744/?&lang=en http://www.teses.usp.br/teses/disponiveis/87/87131/tde-05022013-092744/?&lang=en Myostatin is a negative regulator agent of muscle growth. In the testosterone supplementation therapy we observed decreased myostatin expression. The aim of this project is to determine the influence of anabolic steroids in the expression of myostatin gene in normal C57BL mice and in the dystrophic model Largemyd. We used the Real Time PCR assay to determine the relative expression of genes. Animals treated only with steroids presented significant increase in body mass and Largemyd showed improvement... tde@sc.usp.br (Santos, André Luís Fernandes dos) Doctoral Thesis Fri, 22 Feb 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/87/87131/tde-05022013-091331/?&lang=en http://www.teses.usp.br/teses/disponiveis/87/87131/tde-05022013-091331/?&lang=en It was intended in this study verify the safety and efficacy of the transplantation of adipose derived stem cells (ASC) in rats with ulcerative colitis induced by trinitrobenzenosulfonico acid (TNBS). The cell population was isolated from the adipose tissue by mechanical dissociation and cultured. The cell behavior was verified by the morphology, proliferation, viability, ability to adhere to the plastic surface and parameters of osteogenic differentiation, adipogenic and chondrogenic. The animals... tde@sc.usp.br (Marcelino, Monica Yonashiro) Master's Dissertation Fri, 22 Feb 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/87/87131/tde-05022013-084410/?&lang=en http://www.teses.usp.br/teses/disponiveis/87/87131/tde-05022013-084410/?&lang=en Leptospirosis is a worldwide important zoonosis caused by bacteria of the genus Leptospira. In Brazil, most cases is caused by L. interrogans serovar Copenhageni. Our goal was to identify leptospiras adhesins by phage display technique. Libraries of genomic fragments resulted in the identification of ligands with affinity for leptospiras hamster tissues. Screening these libraries against heparan sulfate proteoglycan (HSPG) identified the proteins LigA and LigB. Recombinant proteins were produced... tde@sc.usp.br (Ching, Ana Tung Ching) Doctoral Thesis Fri, 22 Feb 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/87/87131/tde-05022013-083629/?&lang=en http://www.teses.usp.br/teses/disponiveis/87/87131/tde-05022013-083629/?&lang=en The expression of AT1 and AT2 receptors of Angiotensin II (Ang II) in human astrocytomas was associated with a worse prognosis. To investigate the molecular mechanisms of Ang II actions in gliomas, the transcriptomic profile of C6 cells treated with Ang II and antagonists of AT1 and AT2 was evaluated. The differentially expressed genes obtained were submitted to functional enrichment, Venn diagram and transcriptome network analyzes. Ang II treatment caused gene expression changes involved in pro-tumor... tde@sc.usp.br (Azevedo, Hátylas Felype Zaneti de) Master's Dissertation Fri, 22 Feb 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/87/87131/tde-05022013-082130/?&lang=en http://www.teses.usp.br/teses/disponiveis/87/87131/tde-05022013-082130/?&lang=en The aim of this work is develop a strategy to identify functional promoter regions from any genome. The modern sugarcane genome was chosen as a model for the development of this strategy that involves the generation of fragments of DNA and cloning them into expression vectors. These fragments were then screened by a transient expression assay using biolistic particle delivery resulting in the isolation of four clones. Three clones were permanently transformed in rice, and 12 plants were obtained.... tde@sc.usp.br (Kuroki, Mayra Akemi) Master's Dissertation Fri, 22 Feb 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/87/87131/tde-05022013-080751/?&lang=en http://www.teses.usp.br/teses/disponiveis/87/87131/tde-05022013-080751/?&lang=en Phenolic compounds belong to a group of toxic environmental pollutants discharged from the process in many industries such as oil refineries and chemical plants. Although phenol has bactericidal action, some microorganisms acquired the ability to adapt and use this compound as a source of carbon and energy, through the coordinated control of metabolic pathways (catabolic). The expression of these pathways may be regulated by: control mechanisms via a global or specific response, but these mechanisms... tde@sc.usp.br (Gracioso, Louise Hase) Master's Dissertation Tue, 19 Feb 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/87/87131/tde-19022013-150502/?&lang=en http://www.teses.usp.br/teses/disponiveis/87/87131/tde-19022013-150502/?&lang=en Saccharomyces cerevisiae is the chosen organism for fuel ethanol production, presenting adapted to the hostile environment of the fermentation vats. Adaptations like flocculation, d foam formation and biofilm are undesirable phenotypes for the Brazilian process. Hence, the early identification of these characteristics is necessary to minimize yield losses and lower the production cost. Proteins codified by FLO gene family are related with these phenotypes, which are positively regulated by Flo8p... tde@sc.usp.br (Fiqueiredo, Catarina Macedo) Doctoral Thesis Tue, 19 Feb 2013 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/87/87131/tde-26112012-090832/?&lang=en http://www.teses.usp.br/teses/disponiveis/87/87131/tde-26112012-090832/?&lang=en The interaction of diazotrophic bacterium Enterobacter sp. ICB481 in sugarcane plantlets was evaluated in cultures with organic or conventional fertilization. Plantlets were separated into groups: organic, conventional and no fertilization (inoculated or not with the bacterium). After 60 days, the organic and conventional fertilizers presented similar growth parameters for: leaf length, root volume, fresh and dried matter of leaves, concentrations of nitrate, ammonium, amino acids, proteins, sugars... tde@sc.usp.br (Ichiwaki, Simone) Master's Dissertation Tue, 18 Dec 2012 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/87/87131/tde-26112012-094603/?&lang=en http://www.teses.usp.br/teses/disponiveis/87/87131/tde-26112012-094603/?&lang=en Acute respiratory infections (ARI) are the most common infectious diseases in humans and respiratory viruses are the most frequent agents in the etiology of the same. The human coronaviruses (HCoVs) are recognized as a common cause of upper respiratory tract infections and, less commonly, lower respiratory tract. Data from the occurrence of HCoV in the Brazilian population are scarce, despite the high incidence of respiratory infections during the winter. This study aims to evaluate the occurrence... tde@sc.usp.br (Góes, Luiz Gustavo Bentim) Doctoral Thesis Tue, 18 Dec 2012 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/87/87131/tde-13122012-141110/?&lang=en http://www.teses.usp.br/teses/disponiveis/87/87131/tde-13122012-141110/?&lang=en Leptospirosis is a zoonosis caused by pathogenic bacteria from genus Leptospira. Despite its importance, the pathogenicity and virulence remain to be elucidated. The leptospires do not present known proteases able to degrade extracellular matrix, an activity essential for the penetration and dissemination within the hosts. Therefore, we proposed the investigation of the leptospiral interaction with plasminogen/plasmin and its implications for infection. Leptospires capture plasminogen on the surface,... tde@sc.usp.br (Vieira, Mônica Larucci) Doctoral Thesis Tue, 18 Dec 2012 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/87/87131/tde-26112012-104547/?&lang=en http://www.teses.usp.br/teses/disponiveis/87/87131/tde-26112012-104547/?&lang=en Retina is composed of specialized neuronal cells, which in case of damage are unable to regenerate. CTIPD is a promissory source to replace the injured tissue because they can differentiate into almost all cell types. The aim of the study was to evaluate the differentiation potential of CTIPD in retinal neurons. CTIPD were submitted to neurosphere formation and evaluated by the expression of nestin, b-III-tubulin and Pax-6. From the neurosphere was performed the induction of differentiation into... tde@sc.usp.br (Morais, Bruna Pereira de) Master's Dissertation Tue, 18 Dec 2012 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/87/87131/tde-26112012-103224/?&lang=en http://www.teses.usp.br/teses/disponiveis/87/87131/tde-26112012-103224/?&lang=en The oncogenic potential of HPV is based on the capacity of viral oncoproteins E6 and E7 to change cellular cycle leading to immortality and malignancy. The important role of oncoproteins in tumor progression and its interaction with numerous cellular targets have relevance in studies to the development of vaccines and therapies against HPV associated cancers. This study investigated intracellular localization of E6 and E7 HPV16/18 oncoproteins and its possible cellular targets. It showed the presence... tde@sc.usp.br (Kavati, Erica Akemi) Master's Dissertation Tue, 18 Dec 2012 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/87/87131/tde-26112012-095546/?&lang=en http://www.teses.usp.br/teses/disponiveis/87/87131/tde-26112012-095546/?&lang=en Shiga toxin-producing Escherichia coli (STEC) and enterotoxigenic Escherichia coli (ETEC) are worldwide involved in diarrheal diseases and produce the heat-labile (LT) and the Shiga (Stx1 and Stx2) toxins, which, cause injuries to man. The diagnosis is an important tool for the correct treatment and control of outbreaks. Thus, immunochromatographic assays (ICs) for ETEC and STEC based on toxins detection were developed and standardized using polyclonal antibodies-gold conjugated (PAbs) and their... tde@sc.usp.br (Rocha, Letícia Barboza) Doctoral Thesis Tue, 18 Dec 2012 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/87/87131/tde-26112012-093055/?&lang=en http://www.teses.usp.br/teses/disponiveis/87/87131/tde-26112012-093055/?&lang=en Chronic wounds require a long treatment time, resulting in high-cost medical care. Therefore, the search for new natural therapeutic healing substances, non-toxic and easy access to population has gained importance in recent years. The purpose of this study was to evaluate the effectiveness of chitosan-alginate biomembrane on wound healing in rats. Two excisional wounds were performed by punch (1.5 cm diameter) on the dorsal of 40 male Wistar rats. Both wounds of each rat were treated with chitosan-alginate... tde@sc.usp.br (Caetano, Guilherme Ferreira) Master's Dissertation Tue, 18 Dec 2012 00:00:00 -0300 http://www.teses.usp.br/teses/disponiveis/87/87131/tde-26112012-085524/?&lang=en http://www.teses.usp.br/teses/disponiveis/87/87131/tde-26112012-085524/?&lang=en MicroRNAs regulate gene expression. Alterations in microRNA expression are involved in the progression of cancers. MiRNA-7 regulates epidermal growth factor receptor (EGFR) signaling and multiple other genes involved in EGFR-related pathways. Head and neck squamous cell carcinoma (HNSCC) is the six most common cancer in the world, with no consensus on molecular markers for prognosis. We assessed EGFR and miRNA-7 expression levels in HNSCC and tumor-free samples. MiRNA-7 was over-expressed in tumor.... tde@sc.usp.br (Soares, Renata Machado) Master's Dissertation