Biomedical Sciences Institute

Decisional strategies in binary choice tasks from childhood to senescence.

tde@sc.usp.br (Victorino, Camila Gomes) — Tue, 30 Apr 2013 00:00:00 -0300

Studies have shown subjects dont maximize their profits all the time. When confronted with binary sequences, with rewards that show up more in one alternative than another, adult volunteers choose one side as the number of rewards the side shows; instead of maximizing in the side that shows more rewards. However, it was related children maximize. Studies assert to the possibility that adults dont maximize because they are searching for a pattern in the sequence. Four experiments were made with sequences...

Quantitative analysis of enteropathogens in children with and without diarrhea in São Paulo city, SP.

tde@sc.usp.br (Merino, Victor Rafael Castillo) — Thu, 21 Mar 2013 00:00:00 -0300

Diarrhea is a major cause of mortality and morbidity worldwide achieving children < 5 years old, and is second cause of death in emerging countries. Intestinal anaerobes like enterotoxigenic B. fragilis and Clostridium difficile are associated to acute diarrhea, as well as diarreiogenic Escherichia coli, Salmonella spp., Shigella spp. and Yersinia enterocolitica. In this study, it was evaluated quantitatively the presence of enteropathogens directly from fezes. Stools specimens from children with...

Search for genes associated with response to the Montenegro skin test for Leishmania antigens.

tde@sc.usp.br (Pereira, Lucas Campana) — Thu, 21 Mar 2013 00:00:00 -0300

The present study aims, through genetic-epidemiological methods, to identify genes associated with response to Leishmania antigen. Using samples Montenegro skin test through the municipalities of Monte Negro (RO) and Assis Brazil (AC). In the first approach were tested with TaqManÒ and the second GWAS, and association analyzes were performed using SPSS and Plink. No associations were found with five SNPs (MyD88, IL12, IL10, IFNGR1, and NRAMP1). The analysis of genome scan data with filters,...

Targeting the ASP- 2 protein of amastigotes of Trypanosoma cruzi to the DEC205 receptor on the surface of dendritic cells.

tde@sc.usp.br (Rampazo, Eline Vital) — Thu, 21 Mar 2013 00:00:00 -0300

As part of the innate immune system, dendritic cells (DCs) are critical in the process of induction of immunity and peripheral tolerance. These cells offer many receptors on their surface and can be classified in different subsets according to the expression there of. Monoclonal antibodies directed against different cellular receptors have been used successfully to treat diseases such as cancer. In the last ten years has been demonstrated that antigen can be sent directly to the receiver DEC205 present...

Chloroquine resistance in Plasmodium vivax: molecular and phenotypic evaluation in the Brazilian Western Amazon.

tde@sc.usp.br (Rodriguez, Rosa Del Carmen Miluska Vargas) — Thu, 21 Mar 2013 00:00:00 -0300

In this study, we performed a molecular and phenotypic evaluation of isolates of P. vivax from Brazilian Western Amazon. The phenotypic evaluation of chloroquine (CQ) sensitivity was performed using the schizont maturation assay. The molecular evaluation was made by typing of five non-synonymous single nucleotide polymorphisms (SNPs) of the pvmdr-1 gene (A266G, A1498G, A2722C, T3226C and A2927T) and variations of the copy number of this gene. As a result, the CQ susceptibility phenotype in 36 isolates...

Protection or exacerbation of monoclonal antibodies generated against antigens of Paracoccidioides brasiliensis in the experimental infection.

tde@sc.usp.br (Thomaz, Luciana) — Thu, 21 Mar 2013 00:00:00 -0300

In this work we evaluated the protection that the monoclonal antibody (MAb) against Hsp60 and against CMH provides the animals infected with the yeast Paracoccidioides brasiliensis and P. lutzii in prophylactic model. The treatment with the MAbs of IgG2a and IgG2b isotypes were protective, inducing the secretion of IL-12p70, IFN-g and TNF-a, Th1 pattern of immune response. We show by immunogold that Hsp60 and MHC molecules are accessible on the cell. And the monospecific antibody against HCM and...

Effect of the endophytic diazotrophic bacterium Rhizobium sp. ICB503 in the growth of sugarcane (Saccharum sp.).

tde@sc.usp.br (Martinez, Lina Rocio Del Pilar Rada) — Thu, 21 Mar 2013 00:00:00 -0300

The effects of Rhizobium sp. ICB503 on the development of sugarcane plants were observed in experiments carried out in two different seasons. Sugarcane plants were submeted to the treatments: B, inoculated with bacteria , BN inoculated with bacteria and treated with nitrate; N treated with NO3-, and control C, uninoculated and untreated with nitrate (NO3-) Plants were maintained for two months in greenhouse. Monthly, several growth and physiological parameters were analyzed. Better results were...

Effect of the deregulation of the UPR pathway in the expression of cyclin A1 in human B lymphocytes.

tde@sc.usp.br (Pinto, Camila Bonin) — Thu, 21 Mar 2013 00:00:00 -0300

The unfolded protein response (UPR) is a signaling pathway activated by endoplasmic reticulum (ER) stress. Previously we described a patient (Patient P) with Common Variable Immunodeficiency (CVID) whose delayed activation of the UPR associates with accumulation of immunoglobulins and slower rate of proliferation. Our results showed that chronic UPR stress interrupted cell cycling of EBV-B cells through dysruption of the cyclic nature of cyclin A1. This interrption is depend of the cell type and...

Standardization of a model of allergic inflammation by exposure to Aedes aegypti mosquito bites.

tde@sc.usp.br (Barros, Michele Silva de) — Thu, 21 Mar 2013 00:00:00 -0300

During blood feeding, Aedes aegypti female mosquitoes inoculate saliva into the skin of their vertebrate hosts, modulating their immune functions and facilitating disease transmission. Some components presents in the saliva of Ae. aegypti have been described and characterized as allergens. In this study, we developed a model of allergic inflammation in mice naturally exposed to Ae. aegypti mosquito bites. Our data show that the sensitization and subsequent challenged with this mosquito salivary components...

The critical role of TLR/MyD88 signaling in the inhibitory effect of proteases Natterins in cell recruitment.

tde@sc.usp.br (Ferreira, Márcio José) — Thu, 21 Mar 2013 00:00:00 -0300

In this work we evaluated whether Natterins (NTR) the majority proteins present in the venom of Thalassophryne nattereri venomous fish are responsible for the inhibitory action of leukocyte recruitment during inflammation and the molecular mechanisms involved in this process. Using intravital microscopy analysis we treated the animals with NTR and 6 h after we induced the leukocyte rolling by topical of KC, LPS or agonist of PAR-4 on the microcirculation. The data showed that treatment with NTR inhibited...

Immune regulatory properties of adipose-derived mesenchymal stem cells in the treatment of experimental autoimmune diabetes.

tde@sc.usp.br (Bassi, Ênio José) — Thu, 21 Mar 2013 00:00:00 -0300

Adipose-derived mesenchymal stem cells (ADMSCs) display immunosuppressive properties representing a promising therapeutic approach for several autoimmune diseases. The aim of this study was to investigate the immune regulatory properties of allogeneic ADMSCs therapy in T cell-mediated experimental autoimmune diabetes in NOD mice. Diabetic NOD mice (blood glucose > 240 mg/dl) were treated or not with ADMSC at days 0, 7 and 14 and blood glucose was monitored once a week for 12 weeks after treatment....

Comparative analysis of acute and chronic inflammatory processes of the subcutaneous tissue and exudate in mice genetically selected for maximal or minimal...

tde@sc.usp.br (Fernandes, Jussara Gonçalves) — Thu, 21 Mar 2013 00:00:00 -0300

AIRmax and AIRmin mouse lines differ in terms of acute inflammatory response after Biogel injection. The distinct AIR in these lines is well established, however, differences in late or chronic inflammatory response to Biogel were not described yet. Thus, we decided to check if the genetic selection that modified the acute inflammatory response in these lines, also affected the development of a chronic inflammatory response. We found that AIRmax mice had higher cellular influx in the inflammatory...

Study of the Max transcription factor in the adolescent mice hippocampus subjected to a model of prolonged social defeat.

tde@sc.usp.br (Amaral, Camila Ematne do) — Tue, 19 Mar 2013 00:00:00 -0300

Depressive disorders affect 1-6% of adolescents each year around the world, being considered the 3rd leading cause of suicide. Little is known about molecular mechanisms contributing to chronic stress induced depression. This study aimed to establish a model of social defeat in adolescent mice and evaluate the effects on emotional behaviors and on hippocampal Max transcription factor gene and protein expression. Methodology: C57BL/6 male mice were paired to an aggressor in a daily basis for 21 days....

Role of Toll-like receptor 4 in vascular reactivity in spontaneously hypertensive rats.

tde@sc.usp.br (Bomfim, Gisele Facholi) — Tue, 19 Mar 2013 00:00:00 -0300

Our objective was to investigate the role of TLR4 in blood pressure and vascular reactivity in SHR. TLR4 is more expressed in mesenteric resistance arteries from SHR 15 week than in Wistar with 15 and SHR 5 weeks. Wistar and SHR with 15 weeks were treated with anti-TLR4 (1mg/dia) or IgG (control) for 15 days via ip. The expression of TLR4, MyD88, phosphorylation of p38 and NF-kB p65, and IL-6 secretion was lower in SHR TLR4 than in SHR IgG. SHR treated with anti-TLR4 had reduced blood pressure versus...

Drug related problems and pharmaceutical interventions in elderly hospitalized in the University of São Paulo Teaching Hospital's medical ward.

tde@sc.usp.br (Tomassi, Mário Henrique) — Tue, 19 Mar 2013 00:00:00 -0300

Drug related problems (DRPs) include medication errors (MEs) and adverse drug reactions (ADRs). The objective of this study was to analyze, classify, quantify and intervene in DRPs occurred in hospitalized elderly patients. This prospective descriptive study of a case series included 28 patients and 100 random prescriptions to them addressed. The identification of DRPs was carry out by methods of active pharmacovigilance and the pharmaceutical interventions (PIs) were performed directly with the...

The macrophage migration inhibitory factor (MIF) and decidual cells survival. In vitro study.

tde@sc.usp.br (Paris, Adriana Fraga Costa Samos) — Tue, 19 Mar 2013 00:00:00 -0300

Previous studies from our laboratory showed that the maternal-fetal interface in mouse at gestation day 10 exhibits high levels of macrophage inhibiting migration factor (MIF) expressed by trophoblast cells and its receptors (CD44/CD74) by decidual cells, making these cells potential targets for this cytokine action. Among the roles attributed to this cytokine, it can be highlighted the pro inflammatory actions on the immune response and on proliferation and cellular survival processes. In this context,...

Thyroid hormone effects on diferential expression of rat heart genes.

tde@sc.usp.br (Rozanski, Andrei) — Tue, 19 Mar 2013 00:00:00 -0300

Cardiac hypertrophy is observed in response to long-term hyperthyroidism. The molecular basis of cardiac hypertrophy induced by hyperthyroidism remains to be determined. Using microarray approach, the gene expression profile of heart tissue from rats submitted to hyperthyroidism were analysed. MAS5 were found to be the best for our low-level analysis. Sarcomeric hotspots such as thick-filaments, M-band and intercalated disks under thyroid hormone (T3) treatment were identified. T3 induced transient...

Comparative analysis of the immunoregulatory mechanism generated by indoleamine 2,3 dioxygenase (IDO) and interferon-gamma (IFN-g) in maternal-placental...

tde@sc.usp.br (Prado, Karen Matias do) — Tue, 19 Mar 2013 00:00:00 -0300

The mechanism of immunoregulation generated by the catabolism of tryptophan by IDO protects the fetus against maternal immune response. In this study, this mechanism was evaluated in renal transplanted pregnant women and immunosuppressed. We examined the expression and activity of IDO in placental compartments of healthy pregnant women and patients with transplants. IDO and IFN-g producing cells were imunolocalizated in villous and decidual region in both groups analyzed, with changes in cell type...

In vivo effects of two novel arylpyrazole glucocorticoid receptor modulators on skeletal muscle structure and function.

tde@sc.usp.br (Guilherme, João Paulo Limongi França) — Fri, 15 Mar 2013 00:00:00 -0300

In this study, we have tested two new selective modulators named L5 and L7 along with dexamethasone in skeletal muscle structural, functional and molecular aspects. Male Wistar rats were treated with progressive doses of dexamethasone, L5 and L7 for 1 and 7 days. While body weight and food intake were decreased by the dexamethasone treatment in all doses, L5/L7 treatments induced gain in body weight similarly to controls. Muscle weight was decreased by dexamethasone, while L5/L7 were ineffective....

Study of vascular reactivity in rats subjected to intestinal ischemia and reperfusion: mechanisms involved and the protective role of simvastatin.

tde@sc.usp.br (Peres, Emilia Cristina) — Fri, 15 Mar 2013 00:00:00 -0300

This work aimed to evaluate the vascular reactivity and the effect of pre-treatment with simvastatin (SIMV, 20mg/kg, p.o.), under this parameter, in the superior mesenteric artery (SMA) and pulmonary artery (PA) of rats subjected to 45 minutes of ischemia and 2 hours of reperfusion (iIR). In these arteries, it was evaluated the endothelium-dependent and -independent function and the contraction induced by phenylephrine the selective a1-adrenergic agonist, in preparations with and without endothelium...